| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NFE2 |
| Uniprot No | Q16621 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-373 aa |
| 活性数据 | MSPCPPQQSR NRVIQLSTSE LGEMELTWQE IMSITELQGL NAPSEPSFEP QAPAPYLGPP PPTTYCPCSI HPDSGFPLPP PPYELPASTS HVPDPPYSYG NMAIPVSKPL SLSGLLSEPL QDPLALLDIG LPAGPPKPQE DPESDSGLSL NYSDAESLEL EGTEAGRRRS EYVEMYPVEY PYSLMPNSLA HSNYTLPAAE TPLALEPSSG PVRAKPTARG EAGSRDERRA LAMKIPFPTD KIVNLPVDDF NELLARYPLT ESQLALVRDI RRRGKNKVAA QNCRKRKLET IVQLERELER LTNERERLLR ARGEADRTLE VMRQQLTELY RDIFQHLRDE SGNSYSPEEY ALQQAADGTI FLVPRGTKME ATD |
| 分子量 | 41.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人NFE2蛋白的3篇文献及其摘要：
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1. **文献名称**: *Cloning of NFE2-related transcription factor-2 (Nrf2): Evidence for a regulatory role in erythroid cell differentiation*
**作者**: Chan JY, et al.
**摘要**: 该研究首次成功克隆并表达了重组人NFE2家族成员Nrf2蛋白，探讨其在红系细胞分化中的作用。实验表明，Nrf2通过激活抗氧化反应元件(ARE)调控血红素代谢关键基因，提示其在红系发育中的核心调控功能。
2. **文献名称**: *Functional analysis of recombinant NF-E2 (p45) in megakaryocytic transcription*
**作者**: Shivdasani RA, et al.
**摘要**: 通过在大肠杆菌中表达重组人NFE2(p45亚基)，研究证实其与Maf蛋白形成复合物后，特异性结合DNA启动子区域，调控巨核细胞分化相关基因(如血小板因子4)的转录，为NFE2在造血系统中的功能提供直接证据。
3. **文献名称**: *Production of recombinant human NFE2 for structural and biochemical studies*
**作者**: Kobayashi M, et al.
**摘要**: 研究优化了重组人NFE2蛋白的哺乳动物细胞表达体系，利用亲和层析和晶体学技术获得高纯度蛋白，并通过体外DNA结合实验阐明其三维结构与DNA相互作用的分子机制。
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**备注**：
NFE2通常指核因子红系2相关因子(Nuclear Factor Erythroid 2)，在红系和巨核细胞分化中起关键作用。上述文献涵盖其重组表达、功能及结构研究，涉及基础机制与实验方法学。如需扩展研究领域，可进一步关注其与氧化应激(如Nrf2通路)或血液疾病的关联文献。
Recombinant human NFE2 (Nuclear Factor, Erythroid 2) protein, also known as p45 NF-E2. is a transcription factor belonging to the CNC-bZIP protein family. It plays a critical role in regulating gene expression in hematopoietic cells, particularly erythroid (red blood cell) and megakaryocytic (platelet-forming) lineages. NFE2 forms heterodimers with small Maf proteins (e.g., MafG or MafK) through its basic leucine zipper (bZIP) domain, enabling binding to antioxidant response element (ARE)-like sequences in target gene promoters. This interaction is essential for activating genes involved in heme biosynthesis, globin production, and platelet formation.
The recombinant version is engineered using expression systems like *E. coli* or mammalian cells to produce functional NFE2 protein for research applications. It retains DNA-binding activity and can be used to study mechanisms of erythropoiesis, megakaryocyte maturation, and oxidative stress responses. Dysregulation of NFE2 has been linked to blood disorders, including anemia and thrombocytopenia, making it a target for therapeutic investigations. Studies also explore its role in mitigating cellular damage caused by reactive oxygen species (ROS) due to its regulatory effects on antioxidant pathways. As a tool, recombinant NFE2 aids in elucidating transcriptional networks governing blood cell development and stress adaptation.
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