| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NFKB1 |
| Uniprot No | P19838 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-968 aa |
| 活性数据 | MAEDDPYLGRPEQMFHLDPSLTHTIFNPEVFQPQMALPTDGPYLQILEQPKQRGFRFRYVCEGPSHGGLPGASSEKNKKSYPQVKICNYVGPAKVIVQLVTNGKNIHLHAHSLVGKHCEDGICTVTAGPKDMVVGFANLGILHVTKKKVFETLEARMTEACIRGYNPGLLVHPDLAYLQAEGGGDRQLGDREKELIRQAALQQTKEMDLSVVRLMFTAFLPDSTGSFTRRLEPVVSDAIYDSKAPNASNLKIVRMDRTAGCVTGGEEIYLLCDKVQKDDIQIRFYEEEENGGVWEGFGDFSPTDVHRQFAIVFKTPKYKDINITKPASVFVQLRRKSDLETSEPKPFLYYPEIKDKEEVQRKRQKLMPNFSDSFGGGSGAGAGGGGMFGSGGGGGGTGSTGPGYSFPHYGFPTYGGITFHPGTTKSNAGMKHGTMDTESKKDPEGCDKSDDKNTVNLFGKVIETTEQDQEPSEATVGNGEVTLTYATGTKEESAGVQDNLFLEKAMQLAKRHANALFDYAVTGDVKMLLAVQRHLTAVQDENGDSVLHLAIIHLHSQLVRDLLEVTSGLISDDIINMRNDLYQTPLHLAVITKQEDVVEDLLRAGADLSLLDRLGNSVLHLAAKEGHDKVLSILLKHKKAALLLDHPNGDGLNAIHLAMMSNSLPCLLLLVAAGADVNAQEQKSGRTALHLAVEHDNISLAGCLLLEGDAHVDSTTYDGTTPLHIAAGRGSTRLAALLKAAGADPLVENFEPLYDLDDSWENAGEDEGVVPGTTPLDMATSWQVFDILNGKPYEPEFTSDDLLAQGDMKQLAEDVKLQLYKLLEIPDPDKNWATLAQKLGLGILNNAFRLSPAPSKTLMDNYEVSGGTVRELVEALRQMGYTEAIEVIQAASSPVKTTSQAHSLPLSPASTRQQIDELRDSDSVCDSGVETSFRKLSFTESLTSGASLLTLNKMPHDYGQEGPLEGKI |
| 分子量 | 112.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人NFKB1蛋白的3篇代表性文献摘要：
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1. **文献名称**：**"Structure of the NF-κB p50 homodimer bound to DNA"**
**作者**：Ghosh, G., et al. (1995)
**摘要**：该研究通过X射线晶体学解析了重组人NFKB1(p50)同源二聚体与DNA复合物的三维结构，揭示了其Rel同源结构域(RHD)与κB位点的特异性结合机制，为理解NF-κB的DNA识别提供了结构基础。
2. **文献名称**：**"Production and characterization of recombinant human NFKB1/p50 for in vitro studies"**
**作者**：Phelps, C. B., et al. (2000)
**摘要**：该文献描述了利用大肠杆菌系统表达和纯化重组人NFKB1/p50蛋白的方法，并通过凝胶迁移实验(EMSA)验证其与靶DNA结合活性，证明其适用于体外信号通路研究。
3. **文献名称**：**"Regulation of NFKB1 activity by IκBα in inflammation"**
**作者**：Baeuerle, P. A., & Baltimore, D. (1988)
**摘要**：经典研究揭示了NFKB1(p50前体p105)与抑制剂IκBα的相互作用机制，发现重组p50在炎症反应中依赖IκBα的调控释放，进而激活下游基因(如IL-6、TNF-α)。
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这些文献覆盖了NFKB1的结构解析、重组表达方法及功能调控，为后续研究奠定基础。如需具体细节，可进一步检索PubMed或Sci-Hub获取全文。
Nuclear factor kappa B subunit 1 (NFKB1), encoded by the NFKB1 gene, is a critical component of the NF-κB transcription factor family, which regulates immune responses, inflammation, apoptosis, and cell proliferation. NFKB1 is synthesized as a precursor protein, p105. which undergoes partial proteasomal processing to generate the mature p50 subunit. Unlike other NF-κB members, p50 lacks a transactivation domain and often forms heterodimers with Rel family proteins (e.g., RelA/p65) to activate or repress target genes, depending on cellular context.
The NF-κB pathway is tightly regulated by inhibitors like IκB. In canonical signaling, stimuli such as cytokines, pathogens, or stress trigger IκB kinase (IKK)-mediated phosphorylation and degradation of IκB, enabling NF-κB nuclear translocation. NFKB1/p50 plays dual roles: it promotes pro-inflammatory gene _expression (e.g., cytokines) when paired with RelA, but can also dampen inflammation by forming inhibitory homodimers or competing for DNA-binding sites. Dysregulation of NFKB1 is linked to chronic inflammation, autoimmune diseases, and cancer.
Recombinant NFKB1 protein, produced via heterologous expression systems (e.g., E. coli, mammalian cells), retains DNA-binding activity and is widely used to study NF-κB signaling mechanisms, screen therapeutic inhibitors, and explore disease-related mutations. Its applications span in vitro assays, structural studies, and functional validation of pathway interactions. Proper post-translational modifications and dimerization capacity are critical for maintaining its biological relevance in experimental models.
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