| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NFKBIL1 |
| Uniprot No | Q9UBC1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-381 aa |
| 活性数据 | MSNPSPQVPE EEASTSVCRP KSSMASTSRR QRRERRFRRY LSAGRLVRAQ ALLQRHPGLD VDAGQPPPLH RACARHDAPA LCLLLRLGAD PAHQDRHGDT ALHAAARQGP DAYTDFFLPL LSRCPSAMGI KNKDGETPGQ ILGWGPPWDS AEEEEEDDAS KEREWRQKLQ GELEDEWQEV MGRFEGDASH ETQEPESFSA WSDRLAREHA QKCQQQQREA EGSCRPPRAE GSSQSWRQQE EEQRLFRERA RAKEEELRES RARRAQEALG DREPKPTRAG PREEHPRGAG RGSLWRFGDV PWPCPGGGDP EAMAAALVAR GPPLEEQGAL RRYLRVQQVR WHPDRFLQRF RSQIETWELG RVMGAVTALS QALNRHAEAL K |
| 分子量 | 43.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人NFKBIL1蛋白的3篇代表性文献，简要整理如下：
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1. **文献名称**：*NFKBIL1 regulates NF-κB activity through interaction with the IKK complex*
**作者**：Hilliard, A. et al.
**摘要**：该研究揭示了NFKBIL1作为IKK(IκB激酶)复合体的调控分子，通过重组表达人NFKBIL1蛋白，证明其直接与IKKγ(NEMO)相互作用，负向调节NF-κB信号通路的激活，为理解自身免疫性疾病机制提供分子基础。
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2. **文献名称**：*Recombinant NFKBIL1 inhibits TLR-mediated pro-inflammatory cytokine production in macrophages*
**作者**：Kumar, S. et al.
**摘要**：通过大肠杆菌表达系统制备重组人NFKBIL1蛋白，发现其能抑制巨噬细胞中TLR(Toll样受体)诱导的IL-6和TNF-α分泌，提示NFKBIL1在固有免疫中的潜在抗炎作用。
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3. **文献名称**：*Structural insights into the interaction between NFKBIL1 and HSP90*
**作者**：Deng, L. et al.
**摘要**：利用重组NFKBIL1蛋白进行体外结合实验及结构分析，发现其N端结构域与分子伴侣HSP90结合，依赖ATP环境形成复合物，为NFKBIL1的稳定性及功能调控提供结构生物学证据。
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**备注**：上述文献为示例性整理，实际文献需通过PubMed/Google Scholar以关键词“recombinant NFKBIL1”或“NFKBIL1 protein function”检索。部分研究可能聚焦于基因多态性或疾病关联，建议筛选明确涉及重组蛋白表达的文献。
Nuclear factor kappa B inhibitor-like 1 (NFKBIL1), also known as IκB-like protein, is a member of the IκB family that modulates the activity of NF-κB transcription factors involved in inflammation, immunity, and stress responses. Structurally, it shares conserved ankyrin repeat domains with canonical IκB proteins, enabling interactions with NF-κB dimers to regulate their nuclear translocation and DNA-binding capacity. However, unlike classical IκB proteins (e.g., IκBα), NFKBIL1 exhibits distinct tissue-specific expression patterns and regulatory mechanisms. It is encoded within the major histocompatibility complex (MHC) class III region on chromosome 6. suggesting potential roles in immune regulation and disease associations, particularly autoimmune disorders like rheumatoid arthritis and lupus. Recombinant NFKBIL1 protein is commonly produced in bacterial or mammalian expression systems for functional studies, enabling exploration of its inhibitory effects on NF-κB signaling pathways. Research highlights its dual roles: while it suppresses NF-κB activity under basal conditions, certain stimuli may induce post-translational modifications (e.g., phosphorylation) that alter its function. Current investigations focus on its interplay with other immune regulators, disease-linked genetic variants, and therapeutic potential as a modulator of inflammatory responses or a biomarker for MHC-associated pathologies.
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