| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NFKBIZ |
| Uniprot No | Q9BYH8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-718 aa |
| 活性数据 | MIVDKLLDDS RGGEGLRDAA GGCGLMTSPL NLSYFYGASP PAAAPGACDA SCSVLGPSAP GSPGSDSSDF SSASSVSSCG AVESRSRGGA RAERQPVEPH MGVGRQQRGP FQGVRVKNSV KELLLHIRSH KQKASGQAVD DFKTQGVNIE QFRELKNTVS YSGKRKGPDS LSDGPACKRP ALLHSQFLTP PQTPTPGESM EDVHLNEPKQ ESSADLLQNI INIKNECSPV SLNTVQVSWL NPVVVPQSSP AEQCQDFHGG QVFSPPQKCQ PFQVRGSQQM IDQASLYQYS PQNQHVEQQP HYTHKPTLEY SPFPIPPQSP AYEPNLFDGP ESQFCPNQSL VSLLGDQRES ENIANPMQTS SSVQQQNDAH LHSFSMMPSS ACEAMVGHEM ASDSSNTSLP FSNMGNPMNT TQLGKSLFQW QVEQEESKLA NISQDQFLSK DADGDTFLHI AVAQGRRALS YVLARKMNAL HMLDIKEHNG QSAFQVAVAA NQHLIVQDLV NIGAQVNTTD CWGRTPLHVC AEKGHSQVLQ AIQKGAVGSN QFVDLEATNY DGLTPLHCAV IAHNAVVHEL QRNQQPHSPE VQELLLKNKS LVDTIKCLIQ MGAAVEAKDR KSGRTALHLA AEEANLELIR LFLELPSCLS FVNAKAYNGN TALHVAASLQ YRLTQLDAVR LLMRKGADPS TRNLENEQPV HLVPDGPVGE QIRRILKGKS IQQRAPPY |
| 分子量 | 78.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人NFKBIZ蛋白的3篇文献概览，基于领域内研究方向整理：
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1. **文献名称**：*"Recombinant Human NFKBIZ Protein Inhibits NF-κB Activation and Reduces Inflammatory Responses in Macrophages"*
**作者**：S. Li et al.
**摘要**：本研究通过在大肠杆菌中表达并纯化重组人NFKBIZ蛋白，证明其通过结合NF-κB二聚体阻断其核转位，显著抑制LPS诱导的巨噬细胞炎症因子(如TNF-α、IL-6)释放，提示其在抗炎治疗中的潜在应用。
2. **文献名称**：*"Structural and Functional Analysis of Recombinant NFKBIZ in Ovarian Cancer Cells"*
**作者**：H. Nakamura & T. Fujita
**摘要**：利用哺乳动物表达系统获得高纯度重组NFKBIZ蛋白，通过X射线晶体学解析其与p50/p65复合物的结合构象。实验发现过表达NFKBIZ可抑制卵巢癌细胞增殖并增强化疗药物敏感性，揭示其抑癌机制。
3. **文献名称**：*"NFKBIZ Recombinant Protein Attenuates Colitis via Modulation of Intestinal Epithelial TLR4 Signaling"*
**作者**：M. Wang et al.
**摘要**：在小鼠结肠炎模型中，腹腔注射重组NFKBIZ蛋白通过下调肠道上皮细胞TLR4/NF-κB通路，减少结肠组织损伤及促炎因子表达，证实其治疗炎症性肠病(IBD)的可行性。
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**说明**：以上文献为基于NFKBIZ蛋白常见研究方向的模拟概括，实际文献需通过PubMed、Web of Science等平台检索确认。研究焦点多集中于重组蛋白制备技术、NF-κB通路调控机制及疾病治疗潜力。
Nuclear factor kappa B inhibitor zeta (NFKBIZ), also known as IKB-zeta or MAIL, is a member of the IκB family that regulates the NF-κB signaling pathway. This protein contains multiple ankyrin repeat domains, which mediate interactions with NF-κB transcription factors. Unlike classical IκB proteins, NFKBIZ not only inhibits NF-κB activity by sequestering it in the cytoplasm but also acts as a transcriptional co-regulator in the nucleus. It plays a critical role in immune responses, inflammation, and cell proliferation. NFKBIZ expression is rapidly induced by proinflammatory stimuli (e.g., TNF-α, IL-1β) or pathogen-associated molecular patterns (PAMPs), suggesting its involvement in feedback regulation of inflammatory processes.
Structurally, recombinant human NFKBIZ proteins are commonly produced via molecular cloning in mammalian or bacterial expression systems. These recombinant forms retain functional domains for binding NF-κB subunits (e.g., p50. p65) and modulating gene expression. Studies using recombinant NFKBIZ have elucidated its dual role: in the cytoplasm, it forms complexes with NF-κB dimers, preventing nuclear translocation; in the nucleus, it may enhance or suppress specific NF-κB target genes (e.g., IL6. CCL20) depending on cellular context.
Its dysregulation is linked to inflammatory diseases (e.g., psoriasis, rheumatoid arthritis), cancer, and autoimmune disorders. Recombinant NFKBIZ serves as a key tool for dissecting NF-κB signaling complexity and developing therapeutic strategies targeting this pathway.
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