| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NIBP |
| Uniprot No | Q96Q05 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-912 aa |
| 活性数据 | MSVELLRSVNDFLWLGAALEGLCSASVIYHYPGGTGGKSGARRFQGSTLPAEAANRHRPGALTTNGINPDTSTEIGRAKNCLSPEDIIDKYKEAISYYSKYKNAGVIELEACIKAVRVLAIQKRSMEASEFLQNAVYINLRQLSEEEKIQRYSILSELYELIGFHRKSAFFKRVAAMQCVAPSIAEPGWRACYKLLLETLPGYSLSLDPKDFSRGTHRGWAAVQMRLLHELVYASRRMGNPALSVRHLSFLLQTMLDFLSDQEKKDVAQSLENYTSKCPGTMEPIALPGGLTLPPVPFTKLPIVRHVKLLNLPASLRPHKMKSLLGQNVSTKSPFIYSPIIAHNRGEERNKKIDFQWVQGDVCEVQLMVYNPMPFELRVENMGLLTSGVEFESLPAALSLPAESGLYPVTLVGVPQTTGTITVNGYHTTVFGVFSDCLLDNLPGIKTSGSTVEVIPALPRLQISTSLPRSAHSLQPSSGDEISTNVSVQLYNGESQQLIIKLENIGMEPLEKLEVTSKVLTTKEKLYGDFLSWKLEETLAQFPLQPGKVATFTINIKVKLDFSCQENLLQDLSDDGISVSGFPLSSPFRQVVRPRVEGKPVNPPESNKAGDYSHVKTLEAVLNFKYSGGPGHTEGYYRNLSLGLHVEVEPSVFFTRVSTLPATSTRQCHLLLDVFNSTEHELTVSTRSSEALILHAGECQRMAIQVDKFNFESFPESPGEKGQFANPKQLEEERREARGLEIHSKLGICWRIPSLKRSGEASVEGLLNQLVLEHLQLAPLQWDVLVDGQPCDREAVAACQVGDPVRLEVRLTNRSPRSVGPFALTVVPFQDHQNGVHNYDLHDTVSFVGSSTFYLDAVQPSGQSACLGALLFLYTGDFFLHIRFHEDSTSKELPPSWFCLPSVHVCALEAQA |
| 分子量 | 127.3 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人NIBP(NIK and IKKβ-Binding Protein)蛋白的3篇示例文献及其摘要(内容为示例，实际文献需通过数据库核实)：
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1. **文献名称**: *"Expression and purification of recombinant human NIBP in E. coli for functional studies"*
**作者**: Zhang L, et al.
**摘要**: 研究报道了通过大肠杆菌表达系统高效表达重组人NIBP蛋白，并优化纯化流程。通过体外实验验证其结合IKKβ的能力，为NF-κB信号通路调控研究提供工具蛋白。
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2. **文献名称**: *"NIBP modulates NF-κB activation by facilitating TRAF2 ubiquitination in inflammatory response"*
**作者**: Chen H, et al.
**摘要**: 发现重组人NIBP通过与TRAF2相互作用促进其泛素化，从而增强TNF-α诱导的NF-κB通路激活，揭示了NIBP在炎症反应中的分子机制。
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3. **文献名称**: *"Structural insights into the NIBP-IKKβ complex and implications for drug design"*
**作者**: Wang Y, et al.
**摘要**: 利用重组人NIBP蛋白进行结晶和结构解析，揭示其与IKKβ的结合界面，提出靶向NIBP-IKKβ相互作用抑制NF-κB通路的潜在治疗策略。
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如需具体文献，建议通过 **PubMed** 或 **Google Scholar** 检索关键词："recombinant human NIBP", "NIBP and NF-kappaB"。
NIBP (NIK and IKKβ-Binding Protein), also known as TRAF3-interacting protein (T3JAM), is a human protein that plays a regulatory role in the NF-κB signaling pathway, a critical mediator of immune responses, inflammation, and cell survival. Structurally, NIBP contains a cytosolic domain that facilitates interactions with key signaling molecules, including NIK (NF-κB-inducing kinase) and IKKβ (IκB kinase β), which are central to the activation of NF-κB transcription factors. By binding to these kinases, NIBP enhances the phosphorylation and degradation of IκBα, leading to NF-κB nuclear translocation and the initiation of pro-inflammatory gene expression.
Recombinant human NIBP protein is engineered using mammalian expression systems (e.g., HEK293 or CHO cells) to ensure proper post-translational modifications. It is purified via affinity chromatography, retaining biological activity for experimental applications. Researchers utilize this recombinant protein to study NF-κB pathway dynamics, particularly in inflammatory diseases, neurodegenerative disorders, and cancers where dysregulated NF-κB signaling is implicated. Additionally, it serves as a tool for screening therapeutic agents targeting NF-κB-associated pathologies. The development of recombinant NIBP has significantly advanced mechanistic insights into cellular stress responses and immune regulation, bridging translational research and drug discovery.
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