| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NIP30 |
| Uniprot No | Q9GZU8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-254 aa |
| 活性数据 | MDGGDDGNLI IKKRFVSEAE LDERRKRRQE EWEKVRKPED PEECPEEVYD PRSLYERLQE QKDRKQQEYE EQFKFKNMVR GLDEDETNFL DEVSRQQELI EKQRREEELK ELKEYRNNLK KVGISQENKK EVEKKLTVKP IETKNKFSQA KLLAGAVKHK SSESGNSVKR LKPDPEPDDK NQEPSSCKSL GNTSLSGPSI HCPSAAVCIG ILPGLGAYSG SSDSESSSDS EGTINATGKI VSSIFRTNTF LEAP |
| 分子量 | 28.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人NIP30蛋白的示例参考文献(内容基于学术文献常见结构虚构，供参考)：
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1. **文献名称**：*"Recombinant Human NIP30 Protein Inhibits Tumor Cell Proliferation via Epigenetic Regulation"*
**作者**：Zhang L, et al.
**摘要**：研究通过体外表达重组人NIP30蛋白，发现其通过结合组蛋白修饰酶(如HDACs)调控基因沉默，显著抑制肝癌细胞增殖，提示其在癌症治疗中的潜在应用。
2. **文献名称**：*"Structural and Functional Characterization of NIP30 in DNA Damage Response"*
**作者**：Gupta S, et al.
**摘要**：利用X射线晶体学解析重组NIP30的结构，揭示其与DNA损伤修复蛋白ATM/ATR的相互作用，阐明其在维持基因组稳定性中的功能机制。
3. **文献名称**：*"NIP30 Modulates Inflammatory Signaling Pathways through Recombinant Protein Interaction Studies"*
**作者**：Kim H, et al.
**摘要**：研究发现重组NIP30蛋白通过抑制NF-κB信号通路，减少促炎因子释放，为炎症性疾病治疗提供新靶点。
4. **文献名称**：*"High-Yield Expression and Purification of Recombinant NIP30 for Antiviral Assays"*
**作者**：Wang Y, et al.
**摘要**：优化大肠杆菌表达系统生产高纯度NIP30蛋白，实验证实其可抑制HIV-1病毒复制，可能通过干扰病毒整合机制实现。
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注：以上文献为示例性内容，实际研究中请通过PubMed或Web of Science检索真实文献。若NIP30研究较少，建议扩展相关关键词如“NIP30 protein function”或结合具体研究领域检索。
Recombinant human NIP30 (Nuclear Interacting Protein 30) is a 30 kDa protein encoded by the *C1orf123* gene, primarily localized to the nucleus. It contains a conserved N-terminal nuclear localization signal (NLS) and a C-terminal coiled-coil domain, facilitating interactions with nuclear proteins and macromolecular complexes. NIP30 is implicated in transcriptional regulation, chromatin remodeling, and cell cycle control, though its precise molecular mechanisms remain under investigation. Studies suggest its involvement in modulating apoptosis and stress responses, potentially linking it to cancer and neurodegenerative disorders.
Recombinant NIP30 is typically produced in *E. coli* or mammalian expression systems (e.g., HEK293 cells) to ensure proper folding and post-translational modifications. Purification often employs affinity tags (e.g., His-tag) followed by chromatography. Its recombinant form enables functional studies, including protein-protein interaction assays, cellular localization experiments, and in vitro enzymatic activity assessments. As a research tool, it aids in dissecting pathways related to genomic stability, epigenetic regulation, and disease pathogenesis. Current efforts focus on elucidating its role in signaling cascades and therapeutic targeting, highlighting its emerging significance in molecular and translational biomedicine.
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