| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NKD2 |
| Uniprot No | Q969F2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-451 aa |
| 活性数据 | GKLQSKHAA AARKRRESPE GDSFVASAYA SGRKGAEEAE RRARDKQELP NGDPKEGPFR EDQCPLQVAL PAEKAEGREH PGQLLSADDG ERAANREGPR GPGGQRLNID ALQCDVSVEE DDRQEWTFTL YDFDNCGKVT REDMSSLMHT IYEVVDASVN HSSGSSKTLR VKLTVSPEPS SKRKEGPPAG QDREPTRCRM EGELAEEPRV ADRRLSAHVR RPSTDPQPCS ERGPYCVDEN TERRNHYLDL AGIENYTSRF GPGSPPVQAK QEPQGRASHL QARSRSQEPD THAVHHRRSQ VLVEHVVPAS EPAARALDTQ PRPKGPEKQF LKSPKGSGKP PGVPASSKSG KAFSYYLPAV LPPQAPQDGH HLPQPPPPPY GHKRYRQKGR EGHSPLKAPH AQPATVEHEV VRDLPPTPAG EGYAVPVIQR HEHHHHHEHH HHHHHHHFHP S |
| 分子量 | 50.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为3篇关于重组人NKD2蛋白研究的假设性参考文献示例(基于可能的研究方向概览，实际文献需通过PubMed或Google Scholar核实)：
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**1. 文献名称**：Expression and Purification of Recombinant Human NKD2 in E. coli for Functional Studies
**作者**：Zhang Y. et al.
**摘要**：本研究成功构建了在大肠杆菌中表达重组人NKD2蛋白的体系，通过优化诱导条件和亲和层析法纯化，获得了高纯度蛋白。实验证实该重组蛋白在体外有效抑制Wnt/β-catenin信号通路，为后续结构解析及分子机制研究奠定了基础。
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**2. 文献名称**：Structural Insights into NKD2 Interaction with Dishevelled in Wnt Signaling
**作者**：Chen L. & Wang H.
**摘要**：通过X射线晶体学解析了重组人NKD2蛋白与Dvl2(Dishevelled)的复合物结构，揭示了NKD2通过特定结构域竞争性结合Dvl，从而阻断Wnt信号传导的分子机制，为靶向Wnt通路药物开发提供依据。
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**3. 文献名称**：Role of Recombinant NKD2 in Suppressing Gastric Cancer Cell Invasion
**作者**：Kim S. et al.
**摘要**：研究证实外源性重组NKD2蛋白可抑制胃癌细胞的迁移和侵袭能力，其作用与下调基质金属蛋白酶MMP-9及抑制EMT(上皮间质转化)过程相关，提示NKD2在肿瘤治疗中的潜在应用价值。
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**备注**：以上为基于NKD2已知生物学功能的假设性文献概括，真实文献请通过学术数据库(如PubMed)检索关键词：**"recombinant NKD2 protein"**, **"NKD2 Wnt signaling"**, **"NKD2 cancer"** 等。若需具体文献协助，请提供更多研究背景或目标方向。
Recombinant human NKD2 (Naked Cuticle Homolog 2) protein is a genetically engineered version of the natural signaling regulator involved in the Wnt pathway, a critical pathway for embryonic development, tissue homeostasis, and cancer progression. NKD2 acts as a feedback antagonist of Wnt/β-catenin signaling by binding to Dishevelled (Dvl) proteins, disrupting their interaction with key pathway components like Frizzled receptors. This interaction inhibits downstream signaling, preventing excessive activation linked to tumors and developmental disorders.
The recombinant protein is typically produced in bacterial or mammalian expression systems, ensuring high purity and bioactivity for research applications. It often includes tags (e.g., His-tag) for ease of purification and detection. Studies utilize recombinant NKD2 to explore its role in modulating Wnt signaling dynamics, epithelial-mesenchymal transition (EMT), and cellular differentiation. Aberrant NKD2 expression is observed in cancers (e.g., colorectal, gastric), making it a potential biomarker or therapeutic target. Current research also investigates its structural domains, such as the conserved EF-hand and Dvl-binding regions, to elucidate mechanisms of pathway regulation. Overall, recombinant NKD2 serves as a vital tool for dissecting Wnt-related diseases and developing targeted therapies.
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