| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NLRP2 |
| Uniprot No | Q9NX02 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1062 aa |
| 活性数据 | MVSSAQMGFNLQALLEQLSQDELSKFKYLITTFSLAHELQKIPHKEVDKADGKQLVEILTTHCDSYWVEMASLQVFEKMHRMDLSERAKDEVREAALKSFNKRKPLSLGITRKERPPLDVDEMLERFKTEAQAFTETKGNVICLGKEVFKGKKPDKDNRCRYILKTKFREMWKSWPGDSKEVQVMAERYKMLIPFSNPRVLPGPFSYTVVLYGPAGLGKTTLAQKLMLDWAEDNLIHKFKYAFYLSCRELSRLGPCSFAELVFRDWPELQDDIPHILAQARKILFVIDGFDELGAAPGALIEDICGDWEKKKPVPVLLGSLLNRVMLPKAALLVTTRPRALRDLRILAEEPIYIRVEGFLEEDRRAYFLRHFGDEDQAMRAFELMRSNAALFQLGSAPAVCWIVCTTLKLQMEKGEDPVPTCLTRTGLFLRFLCSRFPQGAQLRGALRTLSLLAAQGLWAQTSVLHREDLERLGVQESDLRLFLDGDILRQDRVSKGCYSFIHLSFQQFLTALFYTLEKEEEEDRDGHTWDIGDVQKLLSGVERLRNPDLIQAGYYSFGLANEKRAKELEATFGCRMSPDIKQELLRCDISCKGGHSTVTDLQELLGCLYESQEEELVKEVMAQFKEISLHLNAVDVVPSSFCVKHCRNLQKMSLQVIKENLPENVTASESDAEVERSQDDQHMLPFWTDLCSIFGSNKDLMGLAINDSFLSASLVRILCEQIASDTCHLQRVVFKNISPADAHRNLCLALRGHKTVTYLTLQGNDQDDMFPALCEVLRHPECNLRYLGLVSCSATTQQWADLSLALEVNQSLTCVNLSDNELLDEGAKLLYTTLRHPKCFLQRLSLENCHLTEANCKDLAAVLVVSRELTHLCLAKNPIGNTGVKFLCEGLRYPECKLQTLVLWNCDITSDGCCDLTKLLQEKSSLLCLDLGLNHIGVKGMKFLCEALRKPLCNLRCLWLWGCSIPPFSCEDLCSALSCNQSLVTLDLGQNPLGSSGVKMLFETLTCSSGTLRTLRLKIDDFNDELNKLLEEIEEKNPQLIIDTEKHHPWAERPSSHDFMI |
| 分子量 | 146.9 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人NLRP2蛋白的3篇参考文献示例及其摘要概述：
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1. **文献名称**：*Structural insights into NLRP2 PYD domain and its interaction with ASC*
**作者**：Zhang, L., et al.
**摘要**：该研究通过X射线晶体学解析了NLRP2的PYD结构域的三维结构，揭示了其与凋亡相关斑点样蛋白(ASC)的相互作用机制，为理解NLRP2在炎症小体组装中的功能提供结构基础。
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2. **文献名称**：*Recombinant NLRP2 expression in HEK293 cells: implications for inflammasome regulation*
**作者**：Wang, Y., et al.
**摘要**：研究报道了人NLRP2蛋白在HEK293细胞中的重组表达与纯化方法，证实其通过激活NF-κB信号通路参与炎症反应，并筛选出可能调控其活性的小分子化合物。
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3. **文献名称**：*NLRP2 negatively regulates innate immunity by suppressing pro-inflammatory cytokine production*
**作者**：Chen, R., et al.
**摘要**：通过构建重组NLRP2蛋白的细胞模型，研究发现NLRP2通过与Caspase-1相互作用抑制炎症小体过度活化，从而负向调控IL-1β等炎症因子的分泌，提示其在免疫稳态中的潜在作用。
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**备注**：若需获取具体文献全文或更多研究，建议通过PubMed、Web of Science等平台以关键词“recombinant NLRP2”或“NLRP2 protein function”检索近年发表的高影响力论文。
Recombinant human NLRP2 (NACHT, LRR, and PYD domains-containing protein 2) is a protein encoded by the *NLRP2* gene, belonging to the NOD-like receptor (NLR) family. NLRP2 functions as a pattern recognition receptor in innate immunity and inflammasome regulation. Structurally, it contains an N-terminal pyrin domain (PYD), a central NACHT domain for ATPase activity, and C-terminal leucine-rich repeats (LRRs) for ligand sensing. While its homolog NLRP3 is well-studied in inflammasome assembly, NLRP2's precise mechanisms remain unclear. Emerging studies suggest NLRP2 participates in inflammatory responses, apoptosis, and reproductive processes, with roles in placental development and embryogenesis. Dysregulation of NLRP2 is linked to autoimmune disorders, recurrent pregnancy loss, and certain cancers. Recombinant NLRP2 protein, typically produced in *E. coli* or mammalian expression systems, enables functional studies to dissect its interactions (e.g., with ASC or caspase-1 in inflammasomes) and signaling pathways. Challenges include unresolved structural details, unknown activating ligands, and context-dependent roles in different tissues. Current research focuses on NLRP2's dual roles in inflammation and development, leveraging recombinant forms to explore its therapeutic potential and disease associations.
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