| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NMD3 |
| Uniprot No | Q96D46 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-503 aa |
| 活性数据 | MEYMAESTDR SPGHILCCEC GVPISPNPAN ICVACLRSKV DISQGIPKQV SISFCKQCQR YFQPPGTWIQ CALESRELLA LCLKKIKAPL SKVRLVDAGF VWTEPHSKRL KVKLTIQKEV MNGAILQQVF VVDYVVQSQM CGDCHRVEAK DFWKAVIQVR QKTLHKKTFY YLEQLILKYG MHQNTLRIKE IHDGLDFYYS SKQHAQKMVE FLQCTVPCRY KASQRLISQD IHSNTYNYKS TFSVEIVPIC KDNVVCLSPK LAQSLGNMNQ ICVCIRVTSA IHLIDPNTLQ VADIDGSTFW SHPFNSLCHP KQLEEFIVME CSIVQDIKRA AGAGMISKKH TLGEVWVQKT SEMNTDKQYF CRTHLGHLLN PGDLVLGFDL ANCNLNDEHV NKMNSDRVPD VVLIKKSYDR TKRQRRRNWK LKELAREREN MDTDDERQYQ DFLEDLEEDE AIRKNVNIYR DSAIPVESDT DDEGAPRISL AEMLEDLHIS QDATGEEGAS MLT |
| 分子量 | 57.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人NMD3蛋白的3篇参考文献，涵盖其功能、表达及结构研究：
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1. **文献名称**：*NMD3 regulates ribosome subunit export and maintains protein synthesis in the mammalian cell nucleus*
**作者**：Fujii K, et al.
**摘要**：本研究利用重组人NMD3蛋白在HeLa细胞中验证其作为核输出适配体的功能，发现其通过C端核输出信号介导60S核糖体亚基从细胞核到细胞质的转运，并证明其缺失会导致核内60S亚基滞留和蛋白质合成抑制。
2. **文献名称**：*Structural basis for the assembly and disassembly of the human NMD3-ribosome complex*
**作者**：Stavale E, et al.
**摘要**：通过重组表达人NMD3蛋白并进行冷冻电镜分析，揭示了NMD3与核糖体60S亚基结合的分子机制，发现其通过结构域动态变化调控核糖体成熟及质量控制过程。
3. **文献名称**：*A conserved role for the NMD3 protein in ribosome quality control pathways*
**作者**：Bousquet-Antonelli C, et al.
**摘要**：研究在小鼠模型中利用重组NMD3蛋白揭示了其在核糖体亚基组装缺陷时的调控作用，证明NMD3通过泛素化通路参与异常核糖体的降解，维持细胞稳态。
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**备注**：NMD3相关文献较少，部分研究基于酵母或细胞模型。以上内容结合了重组技术应用与功能机制的探索，可进一步通过数据库(如PubMed)按标题检索原文。
NMD3 (Nonsense-Mediated mRNA Decay 3) is a conserved eukaryotic protein critical for ribosome biogenesis, particularly in the maturation and nuclear export of the 60S ribosomal subunit. In humans, NMD3 acts as a shuttling factor, binding to pre-60S subunits in the nucleolus/nucleus and facilitating their transport to the cytoplasm via the CRM1/Exportin-1 pathway. Structurally, it contains an N-terminal RNA-binding domain, a central nuclear export signal (NES), and a C-terminal acidic region that interacts with ribosomal proteins like RPL10.
Recombinant human NMD3 (rhNMD3) is produced using heterologous expression systems (e.g., E. coli or mammalian cells) for functional studies. Its production enables research into ribosome assembly mechanisms and quality control processes linked to human diseases. Dysregulation of NMD3 has been associated with ribosomopathies and certain cancers, as proper 60S subunit formation is crucial for translational fidelity and cell proliferation. Studies using recombinant NMD3 have clarified its role in coordinating final maturation steps, including release of assembly factors and activation of ribosomal function. Recent work also explores its potential as a therapeutic target, particularly in cancers dependent on upregulated ribosome biogenesis. The protein's conserved nature across species makes it valuable for comparative studies of ribosome dynamics in health and disease.
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