| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NOL4 |
| Uniprot No | O94818 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-638 aa |
| 活性数据 | MESERDMYRQ FQDWCLRTYG DSGKTKTVTR KKYERIVQLL NGSESSSTDN AKFKFWVKSK GFQLGQPDEV RGGGGGAKQV LYVPVKTTDG VGVDEKLSLR RVAVVEDFFD IIYSMHVETG PNGEQIRKHA GQKRTYKAIS ESYAFLPREA VTRFLMSCSE CQKRMHLNPD GTDHKDNGKP PTLVTSMIDY NMPITMAYMK HMKLQLLNSQ QDEDESSIES DEFDMSDSTR MSAVNSDLSS NLEERMQSPQ NLHGQQDDDS AAESFNGNET LGHSSIASGG THSREMGDSN SDGKTGLEQD EQPLNLSDSP LSAQLTSEYR IDDHNSNGKN KYKNLLISDL KMEREARENG SKSPAHSYSS YDSGKNESVD RGAEDLSLNR GDEDEDDHED HDDSEKVNET DGVEAERLKA FNMFVRLFVD ENLDRMVPIS KQPKEKIQAI IDSCRRQFPE YQERARKRIR TYLKSCRRMK RSGFEMSRPI PSHLTSAVAE SILASACESE SRNAAKRMRL ERQQDESAPA DKQCKPEATQ ATYSTSAVPG SQDVLYINGN GTYSYHSYRG LGGGLLNLND ASSSGPTDLS MKRQLATSSG SSSSSNSRPQ LSPTEINAVR QLVAGYRESA AFLLRSADEL ENLILQQN |
| 分子量 | 71.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于重组人NOL4蛋白的参考文献的简要列举：
1. **"Cloning and functional analysis of human NOL4 in DNA damage response"**
*作者：Zhang Y, et al.*
摘要：本研究成功克隆并表达重组人NOL4蛋白，揭示其在DNA损伤修复通路中的作用，发现NOL4通过调控p53蛋白稳定性参与细胞凋亡。
2. **"Recombinant NOL4 interacts with RNA splicing factors in vitro"**
*作者：Lee SM, Kim DH.*
摘要：利用大肠杆菌系统表达纯化重组人NOL4蛋白，通过体外结合实验证实其与多种RNA剪接因子相互作用，提示NOL4可能参与转录后调控。
3. **"NOL4 modulates oxidative stress by regulating mitochondrial genes"**
*作者：Chen L, et al.*
摘要：通过重组NOL4蛋白的功能实验，发现其能抑制活性氧(ROS)积累，并影响线粒体相关基因表达，可能作为氧化应激的潜在治疗靶点。
*注：以上内容为模拟文献，实际研究中建议通过PubMed、Google Scholar等平台以“NOL4 recombinant human”等关键词检索最新论文。*
NOL4 (Nucleolar Protein 4), also known as NLOL4 or Thoc1. is a protein encoded by the *NOL4* gene in humans. It localizes predominantly to the nucleolus, a subnuclear compartment involved in ribosome biogenesis. Structurally, NOL4 contains conserved regions implicated in RNA binding and protein-protein interactions, suggesting roles in RNA metabolism or ribosomal RNA (rRNA) processing. While its precise molecular functions remain under investigation, studies link NOL4 to transcriptional regulation, DNA damage response, and apoptosis. It interacts with proteins like Bcl-2 family members, potentially modulating cell survival pathways.
Recombinant NOL4 protein, produced via expression systems like *E. coli* or mammalian cells, enables functional and structural studies. Its purified form facilitates investigations into binding partners, enzymatic activity, and cellular localization. Researchers utilize recombinant NOL4 to explore its involvement in diseases, particularly cancers, where dysregulation of nucleolar proteins is common. For instance, altered NOL4 expression has been observed in leukemia and solid tumors, implying a context-dependent role in oncogenesis. Additionally, it may serve as a biomarker or therapeutic target. However, challenges persist in fully elucidating its mechanisms due to complex nucleolar dynamics and pleiotropic effects. Ongoing research aims to clarify its contributions to cellular homeostasis and pathology, leveraging recombinant tools to advance diagnostic and therapeutic strategies.
×
