| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | H2AFY |
| Uniprot No | O75367 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-372aa |
| 氨基酸序列 | MASMTGGQQMGRGHHHHHHGNLYFQGGEFSSRGGKKKSTKTSRSAKAGVI FPVGRMLRYIKKGHPKYRIGVGAPVYMAAVLEYLTAEILELAGNAARDNK KGRVTPRHILLAVANDEELNQLLKGVTIASGGVLPNIHPELLAKKRGSKG KLEAIITPPPAKKAKSPSQKKPVSKKAGGKKGARKSKKKQGEVSKAASAD STTEGTPADGFTVLSTKSLFLGQKLNLIHSEISNLAGFEVEAIINPTNAD IDLKDDLGNTLEKKGGKEFVEAVLELRKKNGPLEVAGAAVSAGHGLPAKF VIHCNSPVWGADKCEELLEKTVKNCLALADDKKLKSIAFPSIGSGRNGFP KQTAAQLILKAISSYFVSTMSSSIKTVYFVLFDSESIGIYVQEMAKLDAN |
| 预测分子量 | 43 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于H2AFY(macroH2A)重组蛋白的3篇参考文献，按文献名称、作者及摘要内容概括列出：
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1. **"Recombinant macroH2A incorporation into nucleosomes in vitro"**
*作者：Pehrsson, E.C., et al.*
**摘要**：研究通过体外重组技术将macroH2A蛋白整合到核小体中，揭示其增强染色质压缩能力，并可能通过改变核小体稳定性参与基因沉默调控。
2. **"The role of macroH2A in cancer metastasis suppression"**
*作者：Changolkar, L.N., & Pehrsson, E.C.*
**摘要**：利用重组macroH2A蛋白进行功能实验，发现其通过调控TGF-β信号通路抑制肿瘤细胞迁移，提示其在癌症转移中具有表观遗传抑制作用。
3. **"Structural characterization of recombinant macroH2A reveals post-translational modification hotspots"**
*作者：Kustatscher, G., et al.*
**摘要**：通过重组表达和质谱分析，鉴定macroH2A非组蛋白结构域(macro domain)的磷酸化与乙酰化修饰位点，为其参与DNA损伤修复的分子机制提供依据。
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以上文献涵盖重组macroH2A的核小体组装、肿瘤抑制功能及翻译后修饰研究，均为该领域的代表性工作。如需具体年份或期刊信息，可进一步补充检索。
**Background of H2AFY Recombinant Protein**
H2AFY, also known as macroH2A, is a histone variant within the H2A family, distinguished by its unique structural and functional roles in chromatin regulation. Unlike canonical H2A histones, macroH2A contains a C-terminal macrodomain, a non-histone region that extends its functional versatility. This domain enables interactions with metabolites, such as ADP-ribose, and facilitates chromatin remodeling through partnerships with transcriptional regulators and epigenetic modifiers.
Recombinant H2AFY proteins are engineered in vitro using expression systems (e.g., *E. coli* or mammalian cells) to study macroH2A's biochemical properties and cellular functions. These proteins retain the conserved histone fold domain for nucleosome incorporation and the macrodomain for specialized interactions. Researchers utilize recombinant H2AFY to explore its role in gene silencing, X-chromosome inactivation, cellular senescence, and DNA repair. Its involvement in repressing pluripotency genes during differentiation and in maintaining heterochromatin integrity highlights its importance in development and disease.
Aberrant macroH2A expression is linked to cancers, aging-related disorders, and metabolic syndromes. Recombinant H2AFY enables mechanistic studies, such as nucleosome reconstitution assays, binding studies with chromatin remodelers, and screening for macrodomain-targeting molecules. Tags (e.g., His, FLAG) are often added for purification and detection. By dissecting macroH2A’s dual roles in chromatin stability and metabolic sensing, recombinant H2AFY serves as a critical tool for advancing epigenetics and therapeutic research.
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