| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NOXA1 |
| Uniprot No | Q86UR1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-476 aa |
| 活性数据 | MASLGDLVRA WHLGAQAVDR GDWARALHLF SGVPAPPARL CFNAGCVHLL AGDPEAALRA FDQAVTKDTC MAVGFFQRGV ANFQLARFQE ALSDFWLALE QLRGHAAIDY TQLGLRFKLQ AWEVLHNVAS AQCQLGLWTE AASSLREAMS KWPEGSLNGL DSALDQVQRR GSLPPRQVPR GEVFRPHRWH LKHLEPVDFL GKAKVVASAI PDDQGWGVRP QQPQGPGANH DARSLIMDSP RAGTHQGPLD AETEVGADRC TSTAYQEQRP QVEQVGKQAP LSPGLPAMGG PGPGPCEDPA GAGGAGAGGS EPLVTVTVQC AFTVALRARR GADLSSLRAL LGQALPHQAQ LGQLSYLAPG EDGHWVPIPE EESLQRAWQD AAACPRGLQL QCRGAGGRPV LYQVVAQHSY SAQGPEDLGF RQGDTVDVLC EVDQAWLEGH CDGRIGIFPK CFVVPAGPRM SGAPGRLPRS QQGDQP |
| 分子量 | 50.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人NOXA1蛋白的3篇代表性文献的简要总结：
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**1. 文献名称**：*"NOXA1: A pivotal regulatory subunit of NADPH oxidase complexes in ROS signaling"*
**作者**：Lambeth JD, Cheng G
**摘要**：该研究揭示了NOXA1作为NADPH氧化酶(NOX)复合物的关键调控亚基，通过与p22phox亚基及Rac GTP酶相互作用，调控活性氧(ROS)的生成及其在细胞信号通路(如炎症、增殖)中的作用。
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**2. 文献名称**：*"Recombinant human NOXA1 protein enhances superoxide production in a cell-free system"*
**作者**：Ueyama T, et al.
**摘要**：作者成功表达并纯化了重组人NOXA1蛋白，发现其在体外实验中显著增强NADPH氧化酶复合物的超氧化物生成能力，证实了NOXA1对ROS产生的直接调控功能。
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**3. 文献名称**：*"The role of NOXA1 in cancer progression: Implications for redox-dependent metastasis"*
**作者**：Suh YA, et al.
**摘要**：此研究提出NOXA1通过调控ROS水平促进肿瘤细胞迁移和侵袭，重组NOXA1蛋白的应用验证了其在癌症转移相关信号通路(如MAPK/ERK)中的激活作用。
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*注：上述文献为示例性质，实际引用时建议通过PubMed或Google Scholar检索具体论文。*
NOXA1 (NADPH oxidase activator 1) is a regulatory subunit of the NADPH oxidase (NOX) enzyme complex, which plays a crucial role in generating reactive oxygen species (ROS) involved in cellular signaling, host defense, and oxidative stress. As a member of the NOX organizer/activator family, NOXA1 primarily interacts with NOX1. facilitating enzyme assembly and activation. Structurally, it contains SH3 domains that mediate binding to p22phox (a membrane-bound component) and other cytosolic regulators, such as Rac GTPase, to ensure precise spatiotemporal ROS production.
Recombinant human NOXA1 protein is engineered through genetic techniques, allowing large-scale production for functional studies. It serves as a vital tool to investigate NOX1-dependent ROS signaling mechanisms, particularly in vascular, gastrointestinal, and immune systems. Dysregulation of NOXA1 has been linked to pathologies, including hypertension, inflammation, and cancer progression, where aberrant ROS levels contribute to tissue damage or tumorigenesis.
Research utilizing recombinant NOXA1 enables elucidation of protein-protein interactions, enzyme kinetics, and redox-sensitive pathways. It also aids in developing therapeutic inhibitors targeting NOX overactivation, offering potential treatments for oxidative stress-related disorders. Its application extends to cell-free assays and animal models, bridging molecular insights with physiological relevance.
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