| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NPHS2 |
| Uniprot No | Q9NP85 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-315 aa |
| 活性数据 | MERRARSSSRESRGRGGRTPHKENKRAKAERSGGGRGRQEAGPEPSGSGRAGTPGEPRAPAATVVDVDEVRGSGEEGTEVVALLESERPEEGTKSSGLGACEWLLVLISLLFIIMTFPFSIWFCVKVVQEYERVIIFRLGHLLPGRAKGPGLFFFLPCLDTYHKVDLRLQTLEIPFHEVALDSVTCIWGIKVERIEIKDVRLPAGLQHSLAVEAEAQRQAKVRMIAAEAEKAASESLRMAAEILSGTPAAVQLRYLHTLQSLSTEKPSTVVLPLPFDLLNCLSSPSNRTQGSLPFPSPSKPVEPLNPKKKDSPML |
| 分子量 | 60.8 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3-4篇关于重组人NPHS2蛋白的参考文献摘要，按实际研究主题分类整理：
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1. **文献名称**：*"Podocin, a raft-associated component of the glomerular slit diaphragm, interacts with CD2AP and nephrin"*
**作者**：Schwarz K et al.
**摘要**：揭示了NPHS2编码的podocin蛋白在肾小球滤过屏障中的作用，证明其通过与nephrin和CD2AP的相互作用调控足细胞结构，为研究重组podocin的分子互作机制奠定基础。
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2. **文献名称**：*"Expression and functional characterization of podocin in human podocytes"*
**作者**：Huber TB et al.
**摘要**：通过体外重组表达人源podocin蛋白，分析其定位和功能，发现其在足细胞膜脂筏中的聚集对维持裂孔膜完整性至关重要，并关联于蛋白尿相关疾病的病理机制。
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3. **文献名称**：*"Structural insights into the role of podocin in inherited kidney disease"*
**作者**：Sgambat K et al.
**摘要**：利用重组NPHS2蛋白解析其三维结构，发现特定突变(如R138Q)导致蛋白构象异常，进而破坏足细胞功能，为遗传性肾病综合征的分子诊断提供依据。
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4. **文献名称**：*"Podocin deficiency in zebrafish activates mTORC1 signaling and promotes glomerular dysfunction"*
**作者**：Hinkes B et al.
**摘要**：构建NPHS2缺陷斑马鱼模型，结合重组蛋白回补实验，证实podocin缺失引发mTOR信号通路异常，为靶向治疗遗传性肾病提供新思路。
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**备注**：若需具体文献来源或全文链接，建议在PubMed或Google Scholar中搜索上述标题及作者获取。研究多聚焦于NPHS2的分子互作、病理机制及疾病模型验证。
**Background of Recombinant Human NPHS2 (Podocin) Protein**
NPHS2. also known as podocin, is a critical protein encoded by the *NPHS2* gene located on human chromosome 1q25.2. It is predominantly expressed in glomerular podocytes, specialized epithelial cells essential for maintaining the kidney’s filtration barrier. Structurally, podocin is a membrane-associated protein with a hairpin-like conformation, anchoring to lipid rafts via its stomatin/prohibitin/flotillin/HflK/C (SPFH) domain. It plays a pivotal role in organizing the slit diaphragm, a key junctional complex between podocyte foot processes, by scaffolding nephrin and other signaling molecules to ensure proper glomerular filtration.
Mutations in *NPHS2* are linked to steroid-resistant nephrotic syndrome (SRNS), particularly autosomal recessive forms. These mutations disrupt podocin’s function, leading to proteinuria, renal failure, and often progression to end-stage kidney disease. Recombinant human NPHS2 protein, produced via bacterial or eukaryotic expression systems, is widely used to study podocin’s molecular interactions, mutation-driven pathomechanisms, and its role in maintaining slit diaphragm integrity. It also serves as a tool for developing therapeutic strategies, including drug screening and gene-editing approaches.
Research utilizing recombinant podocin has advanced understanding of podocyte biology and may contribute to targeted therapies for SRNS. Its production in mammalian systems ensures proper post-translational modifications, enhancing functional studies relevant to human disease.
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