关键信息
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基因名
PRPF4
- 应用
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别名
hPrp4; HPRP4P ; PRP4 ; PRP4 homolog; PRP4 pre mRNA processing factor 4 homolog (yeast); PRP4/STK/WD splicing factor; PRP4_HUMAN; Prp4p ; PRPF4; RP70; SNRNP60; U4/U6 small nuclear ribonucleoprotein Prp4; U4/U6 snRNP 60 kDa protein; WD splicing factor Prp4
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
O43172
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表达区间
1-522 aa
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氨基酸序列
MASSRASSTQ ATKTKAPDDL VAPVVKKPHI YYGSLEEKER ERLAKGESGI LGKDGLKAGI EAGNINITSG EVFEIEEHIS ERQAEVLAEF ERRKRARQIN VSTDDSEVKA CLRALGEPIT LFGEGPAERR ERLRNILSVV GTDALKKTKK DDEKSKKSKE EYQQTWYHEG PNSLKVARLW IANYSLPRAM KRLEEARLHK EIPETTRTSQ MQELHKSLRS LNNFCSQIGD DRPISYCHFS PNSKMLATAC WSGLCKLWSV PDCNLLHTLR GHNTNVGAIV FHPKSTVSLD PKDVNLASCA ADGSVKLWSL DSDEPVADIE GHTVRVARVM WHPSGRFLGT TCYDRSWRLW DLEAQEEILH QEGHSMGVYD IAFHQDGSLA GTGGLDAFGR VWDLRTGRCI MFLEGHLKEI YGINFSPNGY HIATGSGDNT CKVWDLRQRR CVYTIPAHQN LVTGVKFEPI HGNFLLTGAY DNTAKIWTHP GWSPLKTLAG HEGKVMGLDI SSDGQLIATC SYDRTFKLWM AE
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分子量
58.4 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
PRPF4, or Pre-mRNA Processing Factor 4, is a critical component involved in the splicing of pre-mRNA, a fundamental process for gene expression regulation in eukaryotic cells. It plays a vital role in the assembly and function of the spliceosome, the complex responsible for removing introns and joining exons in mRNA transcripts. Mutations or dysfunctions in PRPF4 have been implicated in various genetic disorders, particularly those affecting the retina, such as Retinitis Pigmentosa, where abnormal splicing leads to photoreceptor cell degeneration. As a result, the characterization and functional analysis of recombinant PRPF4 protein have gained significant attention in molecular biology and genetics. Researchers aim to understand its structure and interaction mechanisms within the spliceosome, employing techniques such as protein expression in various systems, and biochemical assays to evaluate splicing activity. Further studies on PRPF4 can not only provide insights into its molecular functions but also contribute to developing therapeutic strategies for splicing-related diseases. Thus, the investigation of PRPF4 recombinant protein is a valuable endeavor in both basic research and potential clinical applications.
