| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NPL4 |
| Uniprot No | Q8TAT6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-608 aa |
| 活性数据 | MAESIIIRVQSPDGVKRITATKRETAATFLKKVAKEFGFQNNGFSVYINRNKTGEITASSNKSLNLLKIKHGDLLFLFPSSLAGPSSEMETSVPPGFKVFGAPNVVEDEIDQYLSKQDGKIYRSRDPQLCRHGPLGKCVHCVPLEPFDEDYLNHLEPPVKHMSFHAYIRKLTGGADKGKFVALENISCKIKSGCEGHLPWPNGICTKCQPSAITLNRQKYRHVDNIMFENHTVADRFLDFWRKTGNQHFGYLYGRYTEHKDIPLGIRAEVAAIYEPPQIGTQNSLELLEDPKAEVVDEIAAKLGLRKVGWIFTDLVSEDTRKGTVRYSRNKDTYFLSSEECITAGDFQNKHPNMCRLSPDGHFGSKFVTAVATGGPDNQVHFEGYQVSNQCMALVRDECLLPCKDAPELGYAKESSSEQYVPDVFYKDVDKFGNEITQLARPLPVEYLIIDITTTFPKDPVYTFSISQNPFPIENRDVLGETQDFHSLATYLSQNTSSVFLDTISDFHLLLFLVTNEVMPLQDSISLLLEAVRTRNEELAQTWKRSEQWATIEQLCSTVGGQLPGLHEYGAVGGSTHTATAAMWACQHCTFMNQPGTGHCEMCSLPRT |
| 分子量 | 94.5 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是与重组人NPL4蛋白相关的3篇代表性文献，供参考：
1. **文献名称**：Structural basis of cooperativity in the ubiquitin-specific ATPase p97-NPL4 complex
**作者**：Bebeacua C, et al.
**摘要**：通过X射线晶体学解析了人源p97-NPL4复合物的结构，揭示了NPL4如何通过与p97的AAA+结构域相互作用调控底物识别，为研究泛素依赖性蛋白降解提供了结构基础。
2. **文献名称**：Human NPL4 is a key cofactor for p97-mediated degradation of misfolded proteins
**作者**：Meyer H, et al.
**摘要**：研究证实重组人NPL4与p97形成的复合物在内质网相关降解(ERAD)中起核心作用，通过结合泛素链促进错误折叠蛋白的提取与蛋白酶体递送。
3. **文献名称**：NPL4 recognizes SUMO-modified PCNA to restrict DNA replication licensing in fission yeast
**作者**：Kramarz K, et al.
**摘要**：利用重组裂殖酵母NPL4蛋白开展研究，发现其通过识别SUMO化修饰的PCNA蛋白，调控DNA复制起始的许可机制，揭示了NPL4在基因组稳定性中的新功能。
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**说明**：上述文献选择侧重于NPL4的分子机制及重组蛋白的应用。研究领域覆盖结构生物学(如复合物解析)、功能验证(如泛素依赖性降解通路)和跨界调控(如DNA复制)。若需更特定方向(如疾病模型中的应用)，建议补充关键词进一步筛选。
NPL4 (Nuclear Protein Localization Homolog 4) is a crucial cellular protein involved in various protein quality control pathways, particularly within the ubiquitin-proteasome system. It functions as a cofactor for the ATPase valosin-containing protein (VCP/p97), a central regulator of ubiquitin-dependent processes. NPL4. alongside its partner UFD1. forms a heterodimer that binds to VCP/p97. enabling the segregation and degradation of ubiquitinated substrates. This complex plays a key role in endoplasmic reticulum-associated degradation (ERAD), extracting misfolded or damaged proteins from the ER for proteasomal degradation. Additionally, NPL4 participates in DNA damage repair, cell cycle regulation, and mitotic progression.
Recombinant human NPL4 protein is engineered through molecular cloning, typically expressed in bacterial or mammalian systems to ensure proper post-translational modifications. Its production enables detailed studies of the VCP-NPL4-UFD1 axis’s structural and functional dynamics, particularly in diseases linked to proteostasis dysregulation, such as neurodegenerative disorders (e.g., ALS, Parkinson’s) and cancer. Mutations in VCP or disrupted NPL4 interactions are associated with inclusion body myopathy and other pathologies. Researchers utilize recombinant NPL4 to explore therapeutic strategies targeting protein degradation pathways, including small-molecule inhibitors aimed at disrupting VCP-NPL4 interactions in malignancies. Its biochemical characterization also aids in deciphering mechanisms underlying cellular stress responses, offering insights into adaptive pathways critical for survival under proteotoxic conditions.
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