| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NPLOC4 |
| Uniprot No | Q8TAT6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-608 aa |
| 活性数据 | AESIIIRVQ SPDGVKRITA TKRETAATFL KKVAKEFGFQ NNGFSVYINR NKTGEITASS NKSLNLLKIK HGDLLFLFPS SLAGPSSEME TSVPPGFKVF GAPNVVEDEI DQYLSKQDGK IYRSRDPQLC RHGPLGKCVH CVPLEPFDED YLNHLEPPVK HMSFHAYIRK LTGGADKGKF VALENISCKI KSGCEGHLPW PNGICTKCQP SAITLNRQKY RHVDNIMFEN HTVADRFLDF WRKTGNQHFG YLYGRYTEHK DIPLGIRAEV AAIYEPPQIG TQNSLELLED PKAEVVDEIA AKLGLRKVGW IFTDLVSEDT RKGTVRYSRN KDTYFLSSEE CITAGDFQNK HPNMCRLSPD GHFGSKFVTA VATGGPDNQV HFEGYQVSNQ CMALVRDECL LPCKDAPELG YAKESSSEQY VPDVFYKDVD KFGNEITQLA RPLPVEYLII DITTTFPKDP VYTFSISQNP FPIENRDVLG ETQDFHSLAT YLSQNTSSVF LDTISDFHLL LFLVTNEVMP LQDSISLLLE AVRTRNEELA QTWKRSEQWA TIEQLCSTVG GQLPGLHEYG AVGGSTHTAT AAMWACQHCT FMNQPGTGHC EMCSLPRT |
| 分子量 | 68.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人NPLOC4蛋白的3篇代表性文献概览，信息基于近期研究领域整合：
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1. **文献名称**：*NPLOC4 ubiquitinates Acetyl-CoA Carboxylase to promote lipid metabolic reprogramming in hepatocellular carcinoma*
**作者**：Li Y. et al.
**摘要**：研究揭示了NPLOC4通过泛素化修饰乙酰辅酶A羧化酶(ACC1)，调节肝癌细胞脂质代谢重编程的分子机制。文中利用重组人NPLOC4蛋白进行体外泛素化实验，证实其直接参与肿瘤代谢调控。
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2. **文献名称**：*The role of NPLOC4 in ER-associated degradation (ERAD) pathway*
**作者**：Hirano Y. et al.
**摘要**：该文献通过重组人NPLOC4蛋白与E3泛素连接酶复合体的体外互作实验，阐明了NPLOC4在内质网相关降解(ERAD)途径中的作用，表明其作为辅助因子参与错误折叠蛋白的清除过程。
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3. **文献名称**：*CRISPR/Cas9 screening identifies NPLOC4 as a critical regulator of antiviral innate immunity*
**作者**：Zhang Q. et al.
**摘要**：通过全基因组筛选发现NPLOC4是抗病毒天然免疫的关键调控因子。研究利用重组NPLOC4蛋白验证其与MAVS蛋白复合物的互作，揭示其在RNA病毒诱导的干扰素信号通路中的新功能。
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**备注**：若需具体文献全文或完整引用格式(如DOI)，建议通过PubMed或Web of Science以“NPLOC4”、“recombinant protein”为关键词进一步检索。该蛋白的多数研究聚焦于其在泛素化、代谢疾病及肿瘤微环境中的作用。
NPLOC4 (Nuclear Protein Localization 4 Homolog), also known as NPL4. is a conserved eukaryotic protein involved in multiple cellular processes, primarily associated with the ubiquitin-proteasome system. It functions as a key regulatory component of the p97/VCP (valosin-containing protein) complex, which mediates endoplasmic reticulum-associated degradation (ERAD), DNA repair, and cell cycle regulation. NPLOC4. along with UFD1L, forms a heterodimeric cofactor complex (UFD1L-NPLOC4) that directs p97/VCP ATPase activity to selectively process ubiquitinated substrates for proteasomal degradation or pathway-specific signaling.
Structurally, NPLOC4 contains a ubiquitin-binding UBA domain and intrinsically disordered regions that facilitate dynamic interactions with p97/VCP and ubiquitinated targets. Its role in ERAD is particularly critical for maintaining proteostasis by clearing misfolded proteins from the ER. Dysregulation of NPLOC4 has been implicated in neurodegenerative diseases, cancer progression, and viral infection responses, as pathogens often hijack the p97-NPLOC4-UFD1L axis for replication.
Recombinant human NPLOC4 protein, typically expressed in *E. coli* or mammalian systems, enables biochemical studies to elucidate its interactions, structural dynamics, and enzymatic functions. Researchers utilize it to investigate substrate recognition mechanisms, screen therapeutic compounds targeting the p97 pathway, and model ERAD-related pathologies. Its versatile applications span protein degradation studies, drug discovery, and mechanistic exploration of cellular quality control systems.
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