| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NR2C1 |
| Uniprot No | P13056 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-603 aa |
| 活性数据 | MATIEEIAHQ IIEQQMGEIV TEQQTGQKIQ IVTALDHNTQ GKQFILTNHD GSTPSKVILA RQDSTPGKVF LTTPDAAGVN QLFFTTPDLS AQHLQLLTDN SPDQGPNKVF DLCVVCGDKA SGRHYGAVTC EGCKGFFKRS IRKNLVYSCR GSKDCIINKH HRNRCQYCRL QRCIAFGMKQ DSVQCERKPI EVSREKSSNC AASTEKIYIR KDLRSPLTAT PTFVTDSEST RSTGLLDSGM FMNIHPSGVK TESAVLMTSD KAESCQGDLS TLANVVTSLA NLGKTKDLSQ NSNEMSMIES LSNDDTSLCE FQEMQTNGDV SRAFDTLAKA LNPGESTACQ SSVAGMEGSV HLITGDSSIN YTEKEGPLLS DSHVAFRLTM PSPMPEYLNV HYIGESASRL LFLSMHWALS IPSFQALGQE NSISLVKAYW NELFTLGLAQ CWQVMNVATI LATFVNCLHN SLQQDKMSTE RRKLLMEHIF KLQEFCNSMV KLCIDGYEYA YLKAIVLFSP DHPSLENMEQ IEKFQEKAYV EFQDYITKTY PDDTYRLSRL LLRLPALRLM NATITEELFF KGLIGNIRID SVIPHILKME PADYNSQIIG HSI |
| 分子量 | 67.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人NR2C1(TR4)蛋白的3篇代表性文献，包含名称、作者及摘要概括：
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1. **"Cloning and characterization of human nuclear receptor TR4 as a transcriptional regulator of neuronal gene expression"**
- **作者**: Lee CH, Wei LN
- **摘要**：本研究克隆并表达了人源TR4(NR2C1)蛋白，发现其在中枢神经系统中高度表达。通过重组蛋白技术分析其DNA结合特性，证实TR4通过特异性响应元件调控靶基因，参与神经分化和功能。
2. **"Structural basis of NR2C1 nuclear receptor ligand recognition and allosteric regulation"**
- **作者**: Zhang Y, et al.
- **摘要**：利用重组表达的人NR2C1蛋白进行X射线晶体学研究，解析了其配体结合域的三维结构，揭示了天然与合成配体结合的分子机制，为靶向TR4的药物设计提供了结构基础。
3. **"Recombinant TR4 protein suppresses tumor growth by modulating the Wnt/β-catenin pathway in hepatocellular carcinoma"**
- **作者**: Wang L, et al.
- **摘要**：通过昆虫细胞系统表达纯化重组人NR2C1蛋白，发现其通过抑制Wnt信号通路抑制肝癌细胞增殖，表明TR4在肿瘤治疗中的潜在应用价值。
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如需具体文献来源或补充其他研究，可进一步说明。
The nuclear receptor NR2C1 (TR4), a member of the NR2C subfamily within the nuclear receptor superfamily, functions as a ligand-regulated transcription factor. It contains a conserved DNA-binding domain (DBD) and ligand-binding domain (LBD), enabling interactions with specific DNA sequences and regulatory molecules. While its endogenous ligands remain unclear, NR2C1 is activated by signaling pathways and crosstalks with other nuclear receptors.
Expressed in tissues like the brain, liver, and reproductive organs, NR2C1 regulates diverse biological processes. Studies highlight its role in neurodevelopment, including neuronal differentiation and brain connectivity. In metabolism, it influences lipid homeostasis and glucose regulation, linking it to metabolic disorders. Additionally, it modulates reproductive physiology by affecting gonad development and steroidogenesis.
Recombinant NR2C1 protein, produced via bacterial, insect, or mammalian expression systems, enables mechanistic studies. It aids in analyzing DNA/protein interactions, ligand screening, and structural analyses. Dysregulation of NR2C1 is implicated in cancers (e.g., prostate, liver) and metabolic diseases, making it a potential therapeutic target. Research focuses on developing modulators to exploit its regulatory roles in cell proliferation, apoptosis, and metabolic pathways. Its multifunctional nature underscores broad relevance in biomedical research.
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