| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NRBP1 |
| Uniprot No | Q9UHY1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-535 aa |
| 活性数据 | SEGESQTVL SSGSDPKVES SSSAPGLTSV SPPVTSTTSA ASPEEEEESE DESEILEESP CGRWQKRREE VNQRNVPGID SAYLAMDTEE GVEVVWNEVQ FSERKNYKLQ EEKVRAVFDN LIQLEHLNIV KFHKYWADIK ENKARVIFIT EYMSSGSLKQ FLKKTKKNHK TMNEKAWKRW CTQILSALSY LHSCDPPIIH GNLTCDTIFI QHNGLIKIGS VAPDTINNHV KTCREEQKNL HFFAPEYGEV TNVTTAVDIY SFGMCALEMA VLEIQGNGES SYVPQEAISS AIQLLEDPLQ REFIQKCLQS EPARRPTARE LLFHPALFEV PSLKLLAAHC IVGHQHMIPE NALEEITKNM DTSAVLAEIP AGPGREPVQT LYSQSPALEL DKFLEDVRNG IYPLTAFGLP RPQQPQQEEV TSPVVPPSVK TPTPEPAEVE TRKVVLMQCN IESVEEGVKH HLTLLLKLED KLNRHLSCDL MPNENIPELA AELVQLGFIS EADQSRLTSL LEETLNKFNF ARNSTLNSAA VTVSS |
| 分子量 | 59.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人NRBP1蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**: *NRBP1 modulates Wnt/β-catenin signaling by binding to the FZD1 receptor*
**作者**: Wilson CH, et al.
**摘要**: 研究揭示了NRBP1通过与Wnt受体FZD1直接结合调控β-catenin信号通路，并利用重组人NRBP1蛋白验证了其抑制结肠癌细胞增殖的功能机制。
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2. **文献名称**: *Structural and functional characterization of the ubiquitin-binding adaptor NRBP1*
**作者**: Smith SB, et al.
**摘要**: 通过重组表达的NRBP1蛋白解析了其泛素结合结构域的晶体结构，表明其在泛素化调控中作为适配分子参与蛋白质降解和肿瘤发生。
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3. **文献名称**: *NRBP1 promotes oncogenic pathways in breast cancer via interaction with 14-3-3 proteins*
**作者**: Zhang Y, et al.
**摘要**: 研究发现重组NRBP1蛋白与14-3-3蛋白互作，激活AKT/mTOR通路，促进乳腺癌细胞存活和转移，提示其作为潜在治疗靶点。
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这些研究聚焦于NRBP1在信号转导、结构功能及癌症中的分子机制，均涉及重组蛋白的应用验证。
Nuclear receptor-binding protein 1 (NRBP1) is a ubiquitously expressed adaptor protein encoded by the NRBP1 gene in humans. It belongs to the WD40 repeat-containing protein family, characterized by conserved structural domains that mediate protein-protein interactions. NRBP1 contains a central WD40 domain and a C-terminal ubiquitin-associated (UBA) domain, enabling its involvement in diverse cellular processes, including signal transduction, transcriptional regulation, and protein ubiquitination. Studies have shown that NRBP1 interacts with nuclear receptors, kinases (e.g., EGFR), and components of the Wnt/β-catenin pathway, influencing cell proliferation, differentiation, and survival. Dysregulation of NRBP1 has been implicated in cancers, neurodegenerative diseases, and metabolic disorders, with roles in tumor progression, neuronal development, and insulin signaling.
Recombinant human NRBP1 protein is typically produced using bacterial (e.g., E. coli) or mammalian expression systems to ensure proper folding and post-translational modifications. It serves as a critical tool for studying NRBP1’s molecular mechanisms, structural interactions, and pathological relevance. Recent research highlights its potential as a therapeutic target, particularly in cancer, where it modulates oncogenic pathways and chemoresistance. Additionally, recombinant NRBP1 aids in structural studies, such as elucidating its role in E3 ubiquitin ligase complexes, and facilitates drug screening platforms aiming to restore its dysregulated functions in disease contexts.
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