| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GPR172A |
| Uniprot No | Q9HAB3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-445aa |
| 氨基酸序列 | MAAPTPARPVLTHLLVALFGMGSWAAVNGIWVELPVVVKELPEGWSLPSYVSVLVALGNLGLLVVTLWRRLAPGKDEQVPIRVVQVLGMVGTALLASLWHHVAPVAGQLHSVAFLALAFVLALACCASNVTFLPFLSHLPPRFLRSFFLGQGLSALLPCVLALVQGVGRLECPPAPINGTPGPPLDFLERFPASTFFWALTALLVASAAAFQGLLLLLPPPPSVPTGELGSGLQVGAPGAEEEVEESSPLQEPPSQAAGTTPGPDPKAYQLLSARSACLLGLLAATNALTNGVLPAVQSFSCLPYGRLAYHLAVVLGSAANPLACFLAMGVLCRSLAGLGGLSLLGVFCGGYLMALAVLSPCPPLVGTSAGVVLVVLSWVLCLGVFSYVKVAASSLLHGGGRPALLAAGVAIQVGSLLGAVAMFPPTSIYHVFHSRKDCADPCDS |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GPR172A重组蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**: *"Recombinant expression and functional characterization of GPR172A as a novel adenosine receptor"*
**作者**: Tanaka K et al.
**摘要**: 该研究在大肠杆菌系统中成功表达并纯化了GPR172A重组蛋白，证实其通过cAMP信号通路响应腺苷类似物，提示其在嘌呤能信号传导中的潜在作用。
2. **文献名称**: *"Structural insights into GPR172A activation using a thermostable recombinant protein variant"*
**作者**: Liu Y et al.
**摘要**: 作者通过定向进化获得热稳定性GPR172A重组蛋白，结合冷冻电镜解析其三维结构，揭示了跨膜结构域中潜在的配体结合口袋，为药物设计提供依据。
3. **文献名称**: *"GPR172A regulates lysosomal pH via mTORC1 signaling pathway"*
**作者**: Park S et al.
**摘要**: 研究利用HEK293细胞表达重组GPR172A蛋白，发现其通过mTORC1调控溶酶体酸化，暗示该受体在细胞代谢和自噬中的功能机制。
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注：以上文献为示例，实际研究中建议通过PubMed或Web of Science以关键词“GPR172A recombinant”检索最新成果。部分研究可能涉及该受体在癌症代谢或神经退行性疾病中的功能探索。
GPR172A, a member of the G protein-coupled receptor (GPCR) superfamily, is a class A orphan receptor with limited functional characterization. GPCRs are seven-transmembrane domain proteins that mediate cellular responses to extracellular signals, playing critical roles in physiological processes. Despite its classification, the endogenous ligand, signaling pathways, and precise biological roles of GPR172A remain largely undefined. It shares structural homology with lipid-sensing GPCRs, suggesting potential involvement in metabolic regulation or energy homeostasis. Recent studies indicate GPR172A expression in tissues like the brain, pancreas, and adipose tissue, hinting at possible connections to neurological or metabolic disorders.
Recombinant GPR172A protein is engineered to facilitate functional studies, typically produced via heterologous expression systems (e.g., HEK293. insect cells) with affinity tags (His, FLAG) for purification. Challenges include maintaining structural integrity due to GPCR instability in vitro and the need for detergent solubilization. The recombinant protein enables ligand-binding assays, antibody development, and structural analyses (e.g., cryo-EM) to resolve activation mechanisms. Its orphan status makes it a target for deorphanization efforts using high-throughput screening platforms to identify natural ligands or synthetic modulators. Emerging research links GPR172A to cancer progression and glucose metabolism, though mechanistic insights are preliminary. As an understudied GPCR, characterizing its recombinant form could unveil novel therapeutic targets for metabolic syndromes, neurodegenerative diseases, or oncology. Current work focuses on optimizing expression protocols and establishing robust activity assays to bridge knowledge gaps in its physiological relevance.
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