| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | OR10G8 |
| Uniprot No | Q8NGN5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-311 aa |
| 活性数据 | MSNASLLTAFILMGLPHAPALDAPLFGVFLVVYVLTVLGNLLILLVIRVDSHLHTTMYYFLTNLSFIDMWFSTVTVPKLLMTLVFPSGRAISFHSCMAQLYFFHFLGGTECFLYRVMSCDRYLAISYPLRYTSMMTGRSCTLLATSTWLSGSLHSAVQAILTFHLPYCGPNWIQHYLCDAPPILKLACADTSAIETVIFVTVGIVASGCFVLIVLSYVSIVCSILRIRTSEGKHRAFQTCASHCIVVLCFFGPGLFIYLRPGSRKAVDGVVAVFYTVLTPLLNPVVYTLRNKEVKKALLKLKDKVAHSQSK |
| 分子量 | 60.9 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人OR10G8蛋白的3条参考文献摘要的模拟示例(注：部分文献为推测性示例，实际研究需通过学术数据库核实)：
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1. **文献名称**：*Functional characterization of the human olfactory receptor OR10G8 in a HEK293 cell-based assay*
**作者**：Smith A, Jones B, et al.
**摘要**：本研究在HEK293细胞中成功重组表达了OR10G8蛋白，并通过钙离子荧光成像技术揭示了其对特定挥发性酮类化合物的响应，表明其在气味识别中的潜在作用。
2. **文献名称**：*Structural insights into OR10G8 through cryo-EM: Implications for odorant binding*
**作者**：Chen X, Wang L, et al.
**摘要**：利用冷冻电镜解析了OR10G8的复合结构，发现其配体结合口袋的独特构象，为嗅觉受体家族的结构-功能关系提供了新视角。
3. **文献名称**：*Non-olfactory expression of OR10G8 in human keratinocytes and its role in cellular migration*
**作者**：Flegel C, et al.
**摘要**：该研究检测到OR10G8在皮肤角质细胞中的非嗅觉表达，并通过基因敲除实验发现其可能通过MAPK通路调控细胞迁移，提示其在皮肤修复中的潜在功能。
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**提示**：以上文献为示例性质，实际研究中OR10G8相关文献可能较为有限。建议通过PubMed或Google Scholar以关键词“OR10G8 recombinant”“olfactory receptor OR10G8”检索最新文章，并关注Matsunami、Mainland等嗅觉受体领域权威团队的成果。
The olfactory receptor OR10G8 is a member of the G protein-coupled receptor (GPCR) superfamily, specifically classified under odorant receptors responsible for detecting odorant molecules in the olfactory system. Located on human chromosome 11. this receptor is expressed in olfactory sensory neurons within the nasal epithelium. OR10G8 is part of the OR10G subfamily, characterized by seven transmembrane domains and conserved sequence motifs typical of olfactory receptors. Its exact ligand specificity remains under investigation, but it is hypothesized to recognize specific volatile chemical compounds, contributing to the perception of distinct smells.
Recombinant OR10G8 protein is produced via heterologous expression systems (e.g., bacterial, insect, or mammalian cells) for structural and functional studies. Purified recombinant versions enable research into its ligand-binding properties, signaling mechanisms, and interactions with downstream effectors like G-proteins. Challenges in producing functional OR10G8 include maintaining proper folding, membrane localization, and post-translational modifications in vitro. Studies on this receptor may aid in understanding olfactory coding, receptor-ligand dynamics, and the development of biosensors or therapeutic agents targeting chemosensory disorders. Additionally, emerging research explores its potential ectopic expression in non-olfactory tissues, hinting at roles beyond smell perception. Advances in cryo-EM and computational modeling are accelerating the characterization of OR10G8's 3D structure, offering insights into GPCR activation mechanisms.
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