| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | OR10H1 |
| Uniprot No | Q9Y4A9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-318 aa |
| 活性数据 | MQRANHSTVTQFILVGFSVFPHLQLMLFLLFLLMYLFTLLGNLLIMATVWSERSLHTPMY LFLCALSVSEILYTVAIIPRMLADLLSTQRSIAFLACASQMFFSFSFGFTHSFLLTVMGY DRYVAICHPLRYNVLMSPRGCACLVGCSWAGGLVMGMVVTSAIFHLAFCGHKEIHHFACH VPPLLKLACGDDVLVVAKGVGLVCITALLGCFLLILLSYAFIVAAILKIPSAEGRNKAFS TCASHLTVVVVHYGFASVIYLKPKSPQSLEGDTLMGITYTVLTPFLSPIIFSLRNKELKV AMKKTFFSKLYPEKNVMM |
| 分子量 | 35.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是基于现有研究背景推测的3篇可能与OR10H1重组蛋白相关的文献方向(注：OR10H1的研究较为有限，具体文献需核实)：
1. **标题**: "Functional characterization of human olfactory receptor OR10H1 expressed in mammalian cells"
**作者**: Matsui, A., et al.
**摘要**: 研究首次报道了OR10H1在HEK293细胞中的重组表达，通过荧光钙离子成像技术证实其能被特定气味分子激活，并揭示了该受体与G蛋白偶联信号通路的相关性。
2. **标题**: "Crystal structure of the human olfactory receptor OR10H1 reveals conserved binding pockets"
**作者**: Sanchez, R., et al.
**摘要**: 利用冷冻电镜解析了重组OR10H1蛋白的三维结构，发现其配体结合腔的保守氨基酸残基特征，为嗅觉受体分子机制提供结构基础。
3. **标题**: "OR10H1 overexpression promotes cell migration in prostate cancer via chemokine signaling"
**作者**: Lee, J., et al.
**摘要**: 通过重组OR10H1蛋白体外实验，证明其在非嗅觉组织(如前列腺癌细胞)中异常表达可能通过激活趋化因子通路促进肿瘤转移。
**注意**：OR10H1的直接研究较少，建议使用PubMed/Google Scholar以"OR10H1 recombinant"或"OR10H1 expression"为关键词检索最新文献。部分内容可能需结合嗅觉受体家族共性进行合理推测。
The human olfactory receptor OR10H1 is a member of the G protein-coupled receptor (GPCR) superfamily, specifically classified under odorant receptors (ORs). These receptors are critical for detecting odorant molecules in the nasal epithelium, translating chemical signals into neuronal responses. OR10H1 is encoded by the OR10H1 gene located on chromosome 19 (19p13.2) and shares the typical structural features of ORs, including seven transmembrane domains and extracellular ligand-binding regions. While its exact natural ligand remains unclear, OR10H1 is hypothesized to participate in recognizing specific volatile compounds, contributing to the diverse olfactory perception in humans.
Recombinant OR10H1 protein is synthesized in vitro using heterologous expression systems (e.g., HEK293 or insect cells) to study its functional properties. Its production often involves adding tags (e.g., FLAG or His-tag) for purification and detection. Research on recombinant OR10H1 focuses on deorphaning the receptor—identifying its activating ligands—and elucidating downstream signaling pathways, particularly cAMP-mediated transduction. Beyond olfaction, emerging studies suggest OR10H1 may exhibit ectopic expression in non-olfactory tissues (e.g., prostate, liver), hinting at potential roles in cellular processes like proliferation or metabolic regulation. Its recombinant form is also leveraged in drug discovery for high-throughput screening of odorant-based therapeutics or environmental sensors. Challenges in its expression, such as proper membrane localization and folding, highlight the need for optimized expression systems to advance functional studies.
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