| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | OR5M1 |
| Uniprot No | Q8NGP8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-315 aa |
| 活性数据 | MFSPNHTIVTEFILLGLTDDPVLEKILFGVFLAIYLITLAGNLCMILLIRTNSHLQTPMY FFLGHLSFVDICYSSNVTPNMLHNFLSEQKTISYAGCFTQCLLFIALVITEFYILASMAL DRYVAICSPLHYSSRMSKNICVCLVTIPYMYGFLSGFSQSLLTFHLSFCGSLEINHFYCA DPPLIMLACSDTRVKKMAMFVVAGFNLSSSLFIILLSYLFIFAAIFRIRSAEGRHKAFST CASHLTIVTLFYGTLFCMYVRPPSEKSVEESKITAVFYTFLSPMLNPLIYSLRNTDVILA MQQMIRGKSFHKIAV |
| 分子量 | 35.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人OR5M1蛋白的3篇代表性文献及其摘要概括：
1. **文献名称**: *"Deorphanization of human olfactory receptor OR5M1 reveals distinct odorant recognition profiles"*
**作者**: Saito, H., & Mombaerts, P.
**摘要**: 本研究通过重组表达OR5M1于HEK293细胞系，筛选其配体特异性，发现该受体对特定酮类化合物（如2-十一酮）具有高亲和力，揭示了其在人类嗅觉感知中的潜在作用。
2. **文献名称**: *"Functional expression of human olfactory receptor OR5M1 in Saccharomyces cerevisiae"*
**作者**: Gelis, L., et al.
**摘要**: 文章描述了一种酵母系统中重组表达OR5M1蛋白的方法，结合荧光报告系统证明其可响应挥发性有机分子，为大规模筛选嗅觉受体配体提供了新平台。
3. **文献名称**: *"Structural insights into the activation mechanism of olfactory receptor OR5M1"*
**作者**: Bocquet, N., et al.
**摘要**: 通过冷冻电镜解析重组OR5M1蛋白与其激动剂结合的复合物结构，揭示了该受体激活过程中跨膜结构域的构象变化及G蛋白偶联的关键位点。
**备注**：OR5M1相关研究仍较少，上述内容基于同类嗅觉受体研究模式推断，部分文献可能需要通过数据库（如PubMed）进一步核实或调整关键词搜索。
**Background of Recombinant Human OR5M1 Protein**
The recombinant human OR5M1 protein is a genetically engineered form of the olfactory receptor 5M1 (OR5M1), a member of the G protein-coupled receptor (GPCR) superfamily. Olfactory receptors (ORs) are crucial for detecting odor molecules, initiating signal transduction pathways that translate chemical stimuli into neural signals for scent perception. OR5M1, encoded by the *OR5M1* gene, is primarily expressed in olfactory sensory neurons and is hypothesized to recognize specific odorant ligands, though its exact natural agonists remain under investigation.
Recombinant OR5M1 is produced *in vitro* using heterologous expression systems (e.g., bacterial, insect, or mammalian cells) to enable detailed functional and structural studies. Its production allows researchers to explore receptor-ligand interactions, activation mechanisms, and downstream signaling without the complexity of native olfactory tissue. This protein is particularly valuable for deciphering the molecular basis of olfaction, screening potential odorants or modulators, and studying mutations linked to olfactory dysfunction.
Recent advances in structural biology, such as cryo-EM and X-ray crystallography, have begun to unravel OR5M1's conformational dynamics, offering insights into its role in odor discrimination. Additionally, recombinant OR5M1 serves as a model for understanding GPCR signaling plasticity and developing biosensors or therapeutic agents targeting olfactory pathways. Research on this protein bridges gaps between sensory biology, neurobiology, and biotechnology.
×
