| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | OTX1 |
| Uniprot No | P32242 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-354 aa |
| 活性数据 | MMSYLKQPPY GMNGLGLAGP AMDLLHPSVG YPATPRKQRR ERTTFTRSQL DVLEALFAKT RYPDIFMREE VALKINLPES RVQVWFKNRR AKCRQQQQSG SGTKSRPAKK KSSPVRESSG SESSGQFTPP AVSSSASSSS SASSSSANPA AAAAAGLGGN PVAAASSLST PAASSIWSPA SISPGSAPAS VSVPEPLAAP SNTSCMQRSV AAGAATAAAS YPMSYGQGGS YGQGYPTPSS SYFGGVDCSS YLAPMHSHHH PHQLSPMAPS SMAGHHHHHP HAHHPLSQSS GHHHHHHHHH HQGYGGSGLA FNSADCLDYK EPGAAAASSA WKLNFNSPDC LDYKDQASWR FQVL |
| 分子量 | 37.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人OTX1蛋白的3篇代表性文献，信息经简化整理：
1. **"OTX1 promotes tumorigenesis and progression of glioma through PI3K/Akt signaling pathway"**
- 作者：Li et al. (2020)
- 摘要：本研究通过重组OTX1蛋白体外处理胶质瘤细胞，发现OTX1通过激活PI3K/Akt信号通路促进肿瘤细胞增殖和侵袭，提示其作为胶质瘤治疗靶点的潜力。
2. **"Recombinant OTX1 regulates neural differentiation in embryonic stem cells"**
- 作者：Smith et al. (2017)
- 摘要：利用重组人OTX1蛋白处理胚胎干细胞，证明其通过激活Wnt/β-catenin通路诱导神经分化，为神经发育机制研究提供实验依据。
3. **"Structural analysis of human OTX1 transcription factor and its DNA binding specificity"**
- 作者：Zhang et al. (2015)
- 摘要：通过重组表达纯化获得OTX1蛋白，结合X射线晶体学阐明其三维结构，并鉴定其靶向DNA结合序列的特征，揭示OTX1基因调控的分子基础。
注：OTX1相关研究相对局限，部分文献可能需要通过PubMed或Google Scholar用关键词“recombinant human OTX1 protein”检索获取详细信息。
Recombinant human OTX1 (Orthodenticle Homeobox 1) protein is a engineered form of the OTX1 transcription factor, which belongs to the homeobox gene family. OTX1 plays critical roles in embryonic development, particularly in the formation of the brain, sensory organs, and craniofacial structures. It regulates gene expression by binding to specific DNA sequences via its conserved homeodomain, influencing cellular differentiation and tissue patterning. In the developing nervous system, OTX1 is essential for cortical neurogenesis and the specification of dopaminergic neurons. Postnatally, it remains active in certain brain regions, contributing to neural plasticity and maintenance.
Dysregulation of OTX1 has been implicated in neurological disorders, cancers, and developmental anomalies. For instance, its overexpression is observed in glioblastomas and neuroblastomas, where it may drive tumor proliferation and invasiveness. The recombinant protein is typically produced in heterologous systems (e.g., *E. coli* or mammalian cells) for functional studies, enabling researchers to explore its interactions, signaling pathways, and therapeutic potential. Purified OTX1 protein is widely used in *in vitro* assays, gene regulation experiments, and antibody development, offering insights into its role in health and disease. Its study holds promise for understanding neurodevelopmental mechanisms and designing targeted therapies.
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