| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | OXA1L |
| Uniprot No | Q15070 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-113 aa |
| 活性数据 | MAMGLMCGRRELLRLLQSGRRVHSVAGPSQWLGKPLTTRLLFPVAPCCCRPHYLFLAASG PRSLSTSAISFAEVQVQAPPVVAATPSPTAVPEVASGETADVVQTAAEQSFAE |
| 分子量 | 48.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"Human OXA1L is an essential component of the mitochondrial protein import machinery"**
- 作者：Stiburek, L., et al.
- 摘要：研究证实OXA1L蛋白在人线粒体膜中参与多肽的插入与组装，尤其在细胞呼吸链复合物IV的生物合成中不可或缺。
2. **"The role of OXA1L in mitochondrial membrane protein biogenesis"**
- 作者：Hahn, A., et al.
- 摘要：通过体外重组实验，揭示OXA1L通过促进线粒体内膜蛋白质的折叠与整合，维持线粒体氧化磷酸化功能的机制。
3. **"Structural insights into the function of human OXA1L in mitochondrial inner membrane assembly"**
- 作者：Richter-Dennerlein, R., et al.
- 摘要：利用重组OXA1L蛋白的晶体结构解析，阐明其通过特定跨膜结构域介导的膜蛋白组装动态过程。
4. **"OXA1L mutations impair mitochondrial respiratory chain function and are linked to neurodegenerative disorders"**
- 作者：Koch, J., et al.
- 摘要：分析重组OXA1L的突变体，发现其功能异常导致线粒体复合物缺陷，并与人类脑部疾病病理相关。
**Background of Recombinant Human OXA1L Protein**
OXA1L (Oxidase Assembly 1-Like) is a nuclear-encoded mitochondrial protein crucial for the biogenesis and maintenance of the mitochondrial inner membrane. As a member of the OXA protein family, it shares homology with the yeast Oxa1p, a key factor in the assembly of oxidative phosphorylation (OXPHOS) complexes. OXA1L primarily facilitates the insertion of mitochondrial DNA (mtDNA)-encoded proteins, particularly subunits of Complex IV (cytochrome c oxidase) and ATP synthase (Complex V), into the inner membrane. This process is essential for proper mitochondrial respiration and energy production.
Defects in OXA1L are linked to mitochondrial dysfunction, contributing to disorders such as encephalopathy, hypertrophic cardiomyopathy, and Leigh syndrome. Its role extends to mitochondrial membrane dynamics, influencing cristae structure and mitophagy. Recombinant human OXA1L protein, produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), enables *in vitro* studies to dissect its molecular mechanisms, interactomes, and post-translational modifications. Researchers utilize this tool to explore pathogenic mutations, screen therapeutic compounds, and model mitochondrial diseases. Understanding OXA1L's function provides insights into broader mitochondrial biology and potential treatments for OXPHOS-related pathologies.
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