| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | OXGR1 |
| Uniprot No | Q96P68 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-337 aa |
| 活性数据 | MNEPLDYLANASDFPDYAAAFGNCTDENIPLKMHYLPVIYGIIFLVGFPGNAVVISTYIF KMRPWKSSTIIMLNLACTDLLYLTSLPFLIHYYASGENWIFGDFMCKFIRFSFHFNLYSS ILFLTCFSIFRYCVIIHPMSCFSIHKTRCAVVACAVVWIISLVAVIPMTFLITSTNRTNR SACLDLTSSDELNTIKWYNLILTATTFCLPLVIVTLCYTTIIHTLTHGLQTDSCLKQKAR RLTILLLLAFYVCFLPFHILRVIRIESRLLSISCSIENQIHEAYIVSRPLAALNTFGNLL LYVVVSDNFQQAVCSTVRCKVSGNLEQAKKISYSNNP |
| 分子量 | 38.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人OXGR1蛋白的3篇代表性文献的简要列举：
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1. **文献名称**: *OXGR1 is a receptor for mitochondrial metabolite α-ketoglutarate in kidney*
**作者**: He, W., et al.
**摘要**: 该研究鉴定了OXGR1作为α-酮戊二酸（α-KG）的细胞膜受体，利用重组OXGR1蛋白进行体外实验，揭示了其在肾脏中通过Gq蛋白信号通路调控酸碱平衡的作用机制。
2. **文献名称**: *OXGR1 modulates renal tubular inflammation and fibrosis via succinate-mediated signaling*
**作者**: Zhou, Y., et al.
**摘要**: 通过重组OXGR1蛋白的功能研究，论文发现其与琥珀酸（succinate）的相互作用可激活炎症和纤维化通路，提出OXGR1在慢性肾病中作为潜在治疗靶点。
3. **文献名称**: *Structural insights into the activation of OXGR1 by α-ketoglutarate*
**作者**: Liu, X., et al.
**摘要**: 研究通过冷冻电镜解析了重组OXGR1蛋白与α-酮戊二酸结合的复合物结构，阐明了配体识别和受体激活的分子基础，为靶向药物设计提供依据。
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以上文献涵盖了OXGR1的功能验证、病理作用及结构解析，均涉及重组蛋白技术的应用。实际引用时建议通过PubMed或Web of Science核对最新研究。
**Background of Recombinant Human OXGR1 Protein**
OXGR1 (α-ketoglutarate receptor 1), also known as GPR99 or P2Y15, is a G protein-coupled receptor (GPCR) belonging to the purinergic receptor family. It is activated by endogenous metabolites, particularly α-ketoglutarate (α-KG), a key intermediate in the tricarboxylic acid (TCA) cycle, and oxaloacetate. OXGR1 is expressed in various tissues, including the kidney, liver, immune cells, and reproductive organs, where it plays roles in metabolic regulation, inflammation, and cellular homeostasis.
Structurally, OXGR1 contains seven transmembrane domains characteristic of GPCRs and signals primarily through Gq or Gi/o pathways, modulating intracellular calcium levels or inhibiting cAMP production. Its activation by α-KG links cellular metabolism to receptor signaling, suggesting a function as a metabolic sensor. Studies implicate OXGR1 in renal electrolyte balance, macrophage-driven inflammatory responses, and fertility regulation. For instance, in the kidney, it influences bicarbonate reabsorption, while in the immune system, it may regulate chemokine secretion.
Recombinant human OXGR1 protein is engineered using mammalian expression systems (e.g., HEK293 cells) to ensure proper post-translational modifications. This protein is critical for *in vitro* studies to elucidate receptor-ligand interactions, downstream signaling mechanisms, and therapeutic targeting. Dysregulation of OXGR1 is associated with metabolic disorders, chronic kidney disease, and inflammatory conditions, making it a potential target for drug development. Research continues to explore its pathophysiological roles and therapeutic applications in precision medicine.
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