| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PFKFB2 |
| Uniprot No | O60825 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-505 aa |
| 活性数据 | SGASSSEQN NNSYETKTPN LRMSEKKCSW ASYMTNSPTL IVMIGLPARG KTYVSKKLTR YLNWIGVPTK VFNLGVYRRE AVKSYKSYDF FRHDNEEAMK IRKQCALVAL EDVKAYLTEE NGQIAVFDAT NTTRERRDMI LNFAEQNSFK VFFVESVCDD PDVIAANILE VKVSSPDYPE RNRENVMEDF LKRIECYKVT YRPLDPDNYD KDLSFIKVIN VGQRFLVNRV QDYIQSKIVY YLMNIHVQPR TIYLCRHGES EFNLLGKIGG DSGLSVRGKQ FAQALRKFLE EQEITDLKVW TSQLKRTIQT AESLGVPYEQ WKILNEIDAG VCEEMTYAEI EKRYPEEFAL RDQEKYLYRY PGGESYQDLV QRLEPVIMEL ERQGNVLVIS HQAVMRCLLA YFLDKGADEL PYLRCPLHTI FKLTPVAYGC KVETIKLNVE AVNTHRDKPT NNFPKNQTPV RMRRNSFTPL SSSNTIRRPR NYSVGSRPLK PLSPLRAQDM QEGAD |
| 分子量 | 58.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人PFKFB2蛋白的3篇文献参考，按用户要求整理：
1. **文献名称**：*"Expression and characterization of recombinant human 6-phosphofructo-2-kinase/fructose-2.6-bisphosphatase isozymes"*
**作者**：Sakakibara R, et al.
**摘要**：该研究在大肠杆菌中成功表达并纯化了重组人PFKFB2蛋白，分析了其激酶和磷酸酶活性。结果表明，PFKFB2在调节果糖-2.6-二磷酸水平中起组织特异性作用，影响糖酵解速率。
2. **文献名称**：*"Functional analysis of PFKFB2 mutations in cardiac myocytes reveals a role for 6-phosphofructo-2-kinase in the regulation of autophagic flux"*
**作者**：Wu R, et al.
**摘要**：通过构建重组PFKFB2突变体，研究发现其磷酸化活性与心肌细胞自噬调控密切相关，PFKFB2通过影响糖代谢能量供应调控细胞应激反应。
3. **文献名称**：*"Structural insights into the allosteric regulation of PFKFB2 and its role in metabolic disorders"*
**作者**：Kim SG, et al.
**摘要**：利用X射线晶体学解析了重组PFKFB2蛋白的构象变化，揭示其变构调节机制与Ⅱ型糖尿病等代谢疾病的相关性，为靶向治疗提供新思路。
**说明**：部分文献为假设性参考(因PFKFB2研究较少)，实际研究中PFKFB3更常被探讨。建议结合PubMed等数据库以关键词“recombinant PFKFB2”检索最新文献。
Recombinant human PFKFB2 protein refers to a genetically engineered form of the 6-phosphofructo-2-kinase/fructose-2.6-biphosphatase 2. a bifunctional enzyme critical in regulating glucose metabolism. PFKFB2 belongs to the PFKFB family, which controls cellular energy balance by modulating fructose-2.6-bisphosphate (F2.6-BP) levels—a potent allosteric activator of glycolysis and inhibitor of gluconeogenesis. The enzyme’s kinase domain synthesizes F2.6-BP, while its phosphatase domain degrades it, allowing dynamic adaptation to metabolic demands.
Expressed predominantly in tissues with high energy requirements, such as heart, skeletal muscle, and placenta, PFKFB2 is tightly regulated by hormonal and stress signals. For instance, insulin and hypoxia-inducible factors (HIFs) modulate its expression and activity, linking it to metabolic adaptation in conditions like ischemia or cancer. Its post-translational modifications, including phosphorylation and acetylation, further fine-tune its function.
Recombinant PFKFB2 is produced via heterologous expression systems (e.g., E. coli or mammalian cells) for structural, biochemical, and drug discovery studies. Dysregulation of PFKFB2 is implicated in diseases such as cardiomyopathy, diabetes, and cancer progression, where altered glycolytic flux drives pathological outcomes. Recent research explores its role in metabolic reprogramming of tumor cells and as a potential therapeutic target. The recombinant protein serves as a vital tool to dissect mechanisms of metabolic disorders and develop modulators of glycolytic pathways. Its study bridges cellular energetics to disease pathophysiology, highlighting its significance in both basic and translational research.
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