| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PIGO |
| Uniprot No | Q8TEQ8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-454 aa |
| 活性数据 | MDSGEWEVSNQHVLGVDHCGHKHGPHHPEMAKKLSQMDQVIQGLVERLENDTLLVVAGDHGMTTNGDHGGDSELEVSAALFLYSPTAVFPSTPPEEPEVIPQVSLVPTLALLLGLPIPFGNIGEVMAELFSGGEDSQPHSSALAQASALHLNAQQVSRFLHTYSAATQDLQAKELHQLQNLFSKASADYQWLLQSPKGAEATLPTVIAELQQFLRGARAMCIESWARFSLSFLLLHLLAAGIPVTTPGPFTVPWQAVSAWALMATQTFYSTGHQPVFPAIHWHAAFVGFPEGHGSCTWLPALLVGANTFASHLLFAVGCPLLLLWPFLCESQGLRKRQQPPGNEADARVRPEEEEEPLMEMRLRDAPQHFYAALLQLGLKYLFILGIQILACALAASILRRHLMVWKVFAPKFIFEAVGFIVSSVGLLLGIALVMRVDGAVSSWFRQLFLAQQR |
| 分子量 | 75.68 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为基于PIGO蛋白相关研究方向的示例性参考文献格式(注：文献为虚拟示例，真实文献需通过数据库查询获取)：
1. **文献名称**：Structural and Functional Analysis of Human PIGO Protein in GPI Biosynthesis
**作者**：T. Kinoshita et al.
**摘要**：研究阐明了人源PIGO蛋白在GPI锚定合成中的催化机制，通过重组表达解析了其三维结构，揭示其与PIGF形成复合物促进GPI酰化反应的分子基础。
2. **文献名称**：PIGO Deficiency: A Novel Mutation Associated with Infantile Seizures and Developmental Delay
**作者**：A. Almeida et al.
**摘要**：通过全外显子测序发现PIGO基因突变导致GPI锚定缺陷的病例，重组表达突变蛋白证实其功能丧失，为相关神经系统疾病的诊断提供依据。
3. **文献名称**：Recombinant Human PIGO Expression in CHO Cells and Its Role in Glycosylation Defects
**作者**：S. Hirose et al.
**摘要**：利用CHO细胞系统表达重组人PIGO蛋白，分析其在GPI锚定糖基化修复中的作用，为先天性糖基化疾病(CDG)的机制研究提供工具。
4. **文献名称**：CRISPR/Cas9-based PIGO Knockout Model Reveals Its Essentiality in Embryonic Development
**作者**：Y. Wang et al.
**摘要**：构建PIGO敲除细胞模型，结合重组蛋白回补实验证明PIGO在胚胎发育中的必要性，并探讨其与凋亡信号通路的关联。
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*提示：真实文献可通过PubMed/Google Scholar以“PIGO protein”“GPI biosynthesis”等关键词检索，侧重关注分子机制或疾病关联研究。*
Recombinant human PIGO protein is a key component in the biosynthesis of glycosylphosphatidylinositol (GPI) anchors, which are critical for attaching certain proteins to cell membranes. The PIGO gene encodes a transmembrane protein involved in the later stages of GPI anchor synthesis, specifically the transfer of phosphoethanolamine (EtNP) to the third mannose residue of the GPI precursor. This modification is essential for linking GPI-anchored proteins to the membrane, impacting diverse cellular processes such as signal transduction, immune response, and cell adhesion.
Mutations in the PIGO gene are associated with inherited GPI deficiency disorders, including Mabry syndrome, characterized by developmental delays, seizures, and elevated serum alkaline phosphatase. Recombinant PIGO protein is produced via heterologous expression systems (e.g., mammalian cells) to ensure proper post-translational modifications. It serves as a vital tool for studying GPI anchor biology, disease mechanisms, and potential therapeutic strategies. Researchers also use it to reconstitute GPI synthesis pathways in vitro or screen for corrective agents in GPI-deficient models. Its applications extend to diagnostics, enabling the detection of GPI-related abnormalities in genetic and metabolic disorders.
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