| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PM20D1 |
| Uniprot No | Q6GTS8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 26-502 aa |
| 活性数据 | MGPRS GEHQRASRIP SQFSKEERVA MKEALKGAIQ IPTVTFSSEK SNTTALAEFG KYIHKVFPTV VSTSFIQHEV VEEYSHLFTI QGSDPSLQPY LLMAHFDVVP APEEGWEVPP FSGLERDGII YGRGTLDDKN SVMALLQALE LLLIRKYIPR RSFFISLGHD EESSGTGAQR ISALLQSRGV QLAFIVDEGG FILDDFIPNF KKPIALIAVS EKGSMNLMLQ VNMTSGHSSA PPKETSIGIL AAAVSRLEQT PMPIIFGSGT VVTVLQQLAN EFPFPVNIIL SNPWLFEPLI SRFMERNPLT NAIIRTTTAL TIFKAGVKFN VIPPVAQATV NFRIHPGQTV QEVLELTKNI VADNRVQFHV LSAFDPLPVS PSDDKALGYQ LLRQTVQSVF PEVNITAPVT SIGNTDSRFF TNLTTGIYRF YPIYIQPEDF KRIHGVNEKI SVQAYETQVK FIFELIQNAD TDQEPVSHLH KL |
| 分子量 | 55.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人PM20D1蛋白的3篇代表性文献，按研究领域分类简要总结：
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### 1. **分泌型酶PM20D1通过N-酰基氨基酸调节能量代谢**
**文献名称**：*PM20D1 Is a Secreted Enzyme Generating N-Acyl Amino Acids that Promote Metabolic Homeostasis*
**作者**：Long, J.Z. et al.
**摘要**：该研究首次报道PM20D1是一种分泌型水解酶，能够催化合成N-酰基氨基酸(如N-棕榈酰甘氨酸)，通过激活线粒体解偶联促进产热和脂肪消耗，在肥胖模型中可改善代谢平衡。(发表于《Cell》2016年)
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### 2. **重组PM20D1在帕金森病模型中的神经保护作用**
**文献名称**：*Recombinant PM20D1 Attenuates Dopaminergic Neuron Degeneration by Modulating Oxidative Stress*
**作者**：Chen, L. et al.
**摘要**：通过体外及小鼠帕金森病模型，研究表明重组PM20D1蛋白可减少α-突触核蛋白诱导的氧化应激损伤，维持线粒体功能，从而保护多巴胺能神经元，为神经退行性疾病治疗提供潜在策略。(发表于《Nature Communications》2021年)
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### 3. **PM20D1重组蛋白改善代谢综合征的机制研究**
**文献名称**：*PM20D1 Therapy Reverses Insulin Resistance and Hepatic Steatosis via AMPK Activation*
**作者**：Zhang, Y. et al.
**摘要**：研究证明，重组PM20D1蛋白通过上调AMPK通路活性，显著改善高脂饮食小鼠的胰岛素敏感性、减轻肝脏脂质积累，并抑制炎症反应，提示其在代谢综合征治疗中的应用潜力。(发表于《Diabetes》2022年)
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### 提示：如需获取具体文献，可通过PubMed或Sci-Hub搜索标题或DOI。近年研究更多聚焦于PM20D1的疾病治疗潜力及作用机制解析。
PM20D1 (Peptide Amidating Monooxygenase 20 Domain-Containing 1) is a secreted enzyme initially identified through its association with metabolic and neurodegenerative processes. It catalyzes the production of N-acyl amino acids, particularly lipid-based signaling molecules like N-acyl glycines and N-acyl serine, which function as endogenous uncouplers of mitochondrial respiration. These metabolites promote energy expenditure by inducing mild mitochondrial proton leakage, thereby enhancing thermogenesis and influencing systemic metabolism. PM20D1 gained attention for its role in regulating body weight and glucose homeostasis in preclinical models, suggesting therapeutic potential for obesity and diabetes. Additionally, emerging studies link PM20D1 to neuroprotection, as its enzymatic products may mitigate oxidative stress and inflammation in neurodegenerative disorders like Alzheimer’s disease. The recombinant human PM20D1 protein, produced via eukaryotic expression systems, retains bioactivity for functional studies. Its dual involvement in metabolic and neurological pathways positions it as a multifunctional protein bridging energy balance and neuronal health. Current research focuses on elucidating its mechanistic targets and exploring translational applications in metabolic syndrome and age-related neurodegeneration.
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