| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PMCH |
| Uniprot No | P20382 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 22-165 aa |
| 活性数据 | ILLSASKSI RNLDDDMVFN TFRLGKGFQK EDTAEKSVIA PSLEQYKNDE SSFMNEEENK VSKNTGSKHN FLNHGLPLNL AIKPYLALKG SVAFPAENGV QNTESTQEKR EIGDEENSAK FPIGRRDFDM LRCMLGRVYR PCWQV |
| 分子量 | 18.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人PMCH蛋白的示例文献概要(注：以下文献为虚拟示例，实际文献需通过学术数据库检索验证)：
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1. **文献名称**: *Expression and Purification of Recombinant Human Pro-Melanin-Concentrating Hormone (hPMCH) in Escherichia coli*
**作者**: Touriño, C. et al.
**摘要**: 本研究描述了一种在大肠杆菌中高效表达重组人PMCH蛋白的方法，通过优化密码子和纯化步骤(包括亲和层析和尺寸排阻色谱)获得高纯度蛋白。重组蛋白的生物活性经体外受体结合实验验证，为结构功能研究奠定了基础。
2. **文献名称**: *Functional Characterization of Recombinant PMCH in Energy Homeostasis Using Rodent Models*
**作者**: Alonso, R. et al.
**摘要**: 通过哺乳动物细胞(CHO系统)表达重组人PMCH，发现其在小鼠模型中通过下丘脑信号通路显著抑制摄食行为，并促进能量消耗。研究提示PMCH蛋白在肥胖治疗中的潜在应用价值。
3. **文献名称**: *Structural Analysis of Glycosylated PMCH Reveals Enhanced Stability in HEK293 Expression System*
**作者**: Smith, J. et al.
**摘要**: 对比不同表达系统后发现，HEK293细胞表达的重组PMCH具有糖基化修饰，较原核表达产物稳定性更高。糖基化形式展现出更强的体外受体激活能力，为开发治疗剂提供了优化策略。
4. **文献名称**: *PMCH Receptor Binding Dynamics Studied via Fluorescent-Labeled Recombinant Protein*
**作者**: Kim, S. et al.
**摘要**: 利用荧光标记的重组PMCH蛋白分析其与GPCR受体(MCHR1)的相互作用，揭示了pH依赖性结合特性及内吞机制，为神经内分泌疾病的药物靶点研究提供了新见解。
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建议通过 **PubMed** 或 **Web of Science** 检索真实文献(关键词：recombinant PMCH/pro-MCH protein)获取具体信息。
Pro-Melanin-Concentrating Hormone (PMCH) is a precursor protein encoded by the PMCH gene in humans. It plays a critical role in regulating energy homeostasis, appetite, and various neuroendocrine functions. The PMCH gene is primarily expressed in the hypothalamus and encodes a 165-amino acid polypeptide that undergoes post-translational processing to generate multiple bioactive peptides, including Melanin-Concentrating Hormone (MCH) and neuropeptides NEI and NGE. MCH, the most studied derivative, acts via G protein-coupled receptors (MCHR1 and MCHR2) and is involved in modulating feeding behavior, energy balance, stress responses, and sleep-wake cycles. Dysregulation of MCH signaling has been linked to metabolic disorders, obesity, and mood-related conditions.
Recombinant human PMCH protein is engineered using expression systems (e.g., E. coli, mammalian cells) to produce purified, functional PMCH or its derived peptides for research. Its recombinant form enables precise study of PMCH’s physiological mechanisms, receptor interactions, and therapeutic potential. Researchers utilize it to explore MCH’s role in obesity pathways, neuronal circuits, and metabolic integration. Additionally, recombinant PMCH aids in developing diagnostics or therapies targeting MCH-related disorders, leveraging its consistent quality and scalability. This protein remains a vital tool for dissecting neuroendocrine networks and advancing translational studies in metabolic and neurological diseases.
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