| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PMPCA |
| Uniprot No | Q10713 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 34-525 aa |
| 活性数据 | SSGGAYP NIPLSSPLPG VPKPVFATVD GQEKFETKVT TLDNGLRVAS QNKFGQFCTV GILINSGSRY EAKYLSGIAH FLEKLAFSST ARFDSKDEIL LTLEKHGGIC DCQTSRDTTM YAVSADSKGL DTVVALLADV VLQPRLTDEE VEMTRMAVQF ELEDLNLRPD PEPLLTEMIH EAAYRENTVG LHRFCPTENV AKINREVLHS YLRNYYTPDR MVLAGVGVEH EHLVDCARKY LLGVQPAWGS AEAVDIDRSV AQYTGGIAKL ERDMSNVSLG PTPIPELTHI MVGLESCSFL EEDFIPFAVL NMMMGGGGSF SAGGPGKGMF SRLYLNVLNR HHWMYNATSY HHSYEDTGLL CIHASADPRQ VREMVEIITK EFILMGGTVD TVELERAKTQ LTSMLMMNLE SRPVIFEDVG RQVLATRSRK LPHELCTLIR NVKPEDVKRV ASKMLRGKPA VAALGDLTDL PTYEHIQTAL SSKDGRLPRT YRLFR |
| 分子量 | 58.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人PMPCA蛋白的3篇文献摘要示例(内容为虚构，供参考)：
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1. **文献名称**：*Recombinant expression and functional characterization of human mitochondrial processing peptidase α (PMPCA)*
**作者**：Smith J, et al.
**摘要**：该研究报道了通过大肠杆菌系统成功表达和纯化重组人PMPCA蛋白，并证实其在体外具有切割线粒体前体蛋白的活性，为研究其酶学机制提供了工具。
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2. **文献名称**：*Structural insights into PMPCA mutations linked to spinocerebellar ataxia*
**作者**：Li X, et al.
**摘要**：作者解析了重组PMPCA蛋白的晶体结构，发现特定突变会导致其底物结合能力下降，阐明了部分遗传性共济失调疾病的分子机制。
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3. **文献名称**：*PMPCA deficiency disrupts mitochondrial proteostasis and induces neurodegeneration in Drosophila models*
**作者**：Garcia R, et al.
**摘要**：利用重组PMPCA蛋白进行功能回补实验，发现其缺失会导致果蝇线粒体蛋白加工异常，进一步引发神经退行性表型，提示其在神经元稳态中的关键作用。
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如需真实文献，建议通过学术数据库(如PubMed)搜索关键词"recombinant PMPCA"或"human PMPCA protein"。近年研究多聚焦于其酶活调控、疾病关联及结构解析领域。
**Background of Recombinant Human PMPCA Protein**
PMPCA (Peptidase Mitochondrial Processing Alpha subunit) is a nuclear-encoded protein critical for mitochondrial function. Located in the mitochondrial matrix, PMPCA forms the alpha subunit of the mitochondrial processing peptidase (MPP), a heterodimeric enzyme complex responsible for cleaving the N-terminal targeting sequences of precursor proteins imported into mitochondria. This proteolytic processing is essential for activating nuclear-encoded mitochondrial proteins, enabling their proper folding and integration into functional complexes.
Structurally, PMPCA belongs to the M16 family of metallopeptidases and requires zinc ions for catalytic activity. Its partner subunit, PMPCB (beta subunit), complements PMPCA to form the active MPP holoenzyme. Mutations in *PMPCA* are linked to mitochondrial disorders, including cerebellar ataxia and neurodegenerative conditions, due to impaired protein maturation and subsequent mitochondrial dysfunction.
Recombinant human PMPCA protein is produced via heterologous expression systems (e.g., *E. coli* or mammalian cells) for functional studies. It serves as a tool to investigate mitochondrial protein import mechanisms, disease pathogenesis, and potential therapeutic strategies targeting mitochondrial proteostasis. Its *in vitro* applications include enzyme activity assays, structural studies, and screening for modulators of MPP function.
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