| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PMS2CL |
| Uniprot No | Q68D20 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-193 aa |
| 活性数据 | MHAADLEKPM VEKQDQSPSL RTGEEKRDVS ISRLREAFSL RHTTENKPHS PKTPEPRRSP LGQKRGMSSS STSDAISDRG VLRPQKEAVS SSQGPSDPTD RAEVEKDSGH GSTSVDSEGF SIPDTGSHCS SECVASTPGD RGSQEHVDSQ EKAPETDDSF SDVDCHSNQE DTGCKFQVLP QPTNLTSPNT KVF |
| 分子量 | 20.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人PMS2CL蛋白的参考文献及摘要概括：
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1. **文献名称**：*Cloning and Functional Analysis of Recombinant Human PMS2CL Protein in DNA Mismatch Repair*
**作者**：Zhang, Y., et al.
**摘要**：本研究成功在大肠杆菌和哺乳动物细胞中表达并纯化了重组人PMS2CL蛋白。实验表明，PMS2CL可与MLH1蛋白形成复合物，并在体外恢复部分DNA错配修复活性，提示其在修复系统中的潜在作用。
2. **文献名称**：*Biochemical Characterization of PMS2CL and Its Interaction with MLH1*
**作者**：Wang, L., & Li, S.
**摘要**：通过重组表达技术获得人PMS2CL蛋白，发现其与MLH1存在直接结合，且该相互作用依赖PMS2CL的C端结构域。研究为PMS2CL在遗传性癌症相关通路中的机制提供了生化证据。
3. **文献名称**：*Expression and Mutational Profiling of PMS2CL in Colorectal Cancer Models*
**作者**：Smith, J., et al.
**摘要**：利用重组PMS2CL蛋白进行体外酶活分析，发现特定错义突变会显著削弱其核酸内切酶活性，暗示PMS2CL功能异常可能参与结直肠癌发生。
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**注**：上述文献为示例性内容，实际研究中请通过学术数据库(如PubMed、Web of Science)以关键词“PMS2CL recombinant”或“PMS2CL protein function”检索最新文献。若研究较少，可扩展至PMS2家族其他成员(如PMS2)的重组蛋白研究作为参考。
PMS2CL (PMS2 C-terminal like) is a protein encoded by the *PMS2CL* gene, a human-specific pseudogene derived from the *PMS2* gene, a key component of the DNA mismatch repair (MMR) system. Located on chromosome 7q11.23. *PMS2CL* arose through segmental duplication of a portion of the functional *PMS2* gene (chromosome 7p22) and retains partial homology to its C-terminal region. However, *PMS2CL* lacks critical functional domains, including the ATPase and endonuclease motifs required for MMR activity, rendering it nonfunctional in DNA repair.
Recombinant PMS2CL protein is often studied to explore its residual structural or regulatory roles, though its biological significance remains unclear. Research suggests it may interact with MMR-related proteins or influence gene regulation, potentially contributing to genomic stability or disease pathogenesis. Its pseudogene status and truncated structure also make it a model for studying gene duplication and pseudogene evolution.
Interest in PMS2CL extends to cancer biology, as *PMS2* mutations are linked to Lynch syndrome and microsatellite instability. While PMS2CL itself is not directly implicated in repair, altered expression or polymorphisms in *PMS2CL* have been explored for associations with cancer risk or progression. Recombinant variants are utilized in structural and functional assays to dissect its hypothetical roles or evolutionary constraints. Further studies are needed to clarify its physiological relevance.
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