| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PMS2L5 |
| Uniprot No | A8MQ11 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-134 aa |
| 活性数据 | MWGRRRKLRR LNDVTISTCH VSAKVGTRLV FDHDGKIIQK TPYPHPRGTT VSVKQLFSTL PVRHKEFQRN IKKKRACFPF AFCRDCQFLE GSPAMLPVQP AKLTPRSTPP HPCSLEDNVI TVFSSVKNGP GSSR |
| 分子量 | 15.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是基于相关领域研究的假设性参考文献框架，实际文献可能需进一步检索确认：
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### 1. **“Functional characterization of recombinant human PMS2L5 in DNA mismatch repair”**
**作者：** Zhang et al., 2020
**摘要：** 本研究成功在大肠杆菌中表达了重组人PMS2L5蛋白，并验证其与MLH1的相互作用。实验表明PMS2L5可能参与非经典的DNA错配修复路径，但其修复活性较PMS2显著降低，提示其潜在调控作用。
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### 2. **“PMS2L5 overexpression promotes genomic instability in colorectal cancer cells”**
**作者：** Lee & Kim, 2019
**摘要：** 通过哺乳动物细胞系统表达重组PMS2L5.发现其异常表达导致染色体异常分离，并与微卫星不稳定性相关，可能作为癌症生物标志物。
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### 3. **“Structural insights into PMS2L5 and its interaction with ATP”**
**作者：** Müller et al., 2021
**摘要：** 利用X射线晶体学解析重组PMS2L5的ATP结合域结构，揭示其ATP酶活性较PMS2弱，可能通过竞争性抑制参与修复通路调控。
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### 4. **“PMS2L5 variants and their association with Lynch-like syndrome”**
**作者：** Gonzalez-Perez et al., 2018
**摘要：** 筛选Lynch样综合征患者的PMS2L5突变，通过重组蛋白功能实验发现某些突变体导致错配修复缺陷，提示其在遗传性癌症中的作用。
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**注：** 若需具体文献，建议在PubMed或Google Scholar中检索最新研究，或确认蛋白的准确命名(如是否涉及PMS2亚型或数据库标注差异)。部分研究可能以“PMS2同源物”或“错配修复蛋白变体”为关键词。
PMS2L5. a putative recombinant human protein, is a less-characterized member associated with the DNA mismatch repair (MMR) system. Its nomenclature suggests a possible link to the PMS2 (Postmeiotic Segregation Increased 2) protein family, critical for correcting DNA replication errors. However, PMS2L5 is not a canonical MMR component and may represent a pseudogene-derived or lineage-specific variant. The "L5" suffix likely denotes paralogous sequences arising from gene duplication events during evolution.
Recombinant PMS2L5 production typically involves cloning its coding sequence into expression vectors (e.g., E. coli or mammalian systems) to enable functional studies. Limited studies speculate its role in modulating genomic stability or interacting with MMR pathways, but conclusive evidence remains sparse. Its recombinant form is primarily used to investigate structural properties, protein-protein interactions, or potential diagnostic/therapeutic applications in conditions like Lynch syndrome or cancers with microsatellite instability.
Current research focuses on clarifying its biological relevance, distinguishing it from canonical PMS2. and exploring its biomarker potential. Further characterization is needed to resolve whether PMS2L5 actively contributes to DNA repair or represents an evolutionary remnant with regulatory non-coding functions.
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