| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PNMAL1 |
| Uniprot No | Q86V59 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-439 aa |
| 活性数据 | MSKTMAMNLL EDWCRGMEVD IHRSLLVTGI PEDCGQAEIE ETLNGVLSPL GPYRVLNKIF VREENVKAAL IEVGEGVNLS TIPREFPGRG GVWRVVCRDP TQDAEFLKNL NEFLDAEGRT WEDVVRLLQL NHPTLSQNQH QPPENWAEAL GVLLGAVVQI IFCMDAEIRS REEARAQEAA EFEEMAAWAL AAGRKVKKEP GLAAEVGSAL KAETPNNWNA TEDQHEPTKP LVRRAGAKSR SRRKKQKKNS RQEAVPWKKP KGINSNSTAN LEDPEVGDAE SMAISEPIKG SRKPCVNKEE LALKKPMAKC AWKGPREPPQ DARAEAESPG GASESDQDGG HESPPKKKAV AWVSAKNPAP MRKKKKVSLG PVSYVLVDSE DGRKKPVMPK KGPGSRREAS DQKAPRGQQP AEATASTSRG PKAKPEGSPR RATNESRKV |
| 分子量 | 48.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于重组人PNMAL1蛋白的示例文献(注：因该蛋白研究可能存在特异性，以下内容为模拟虚构，仅供参考)：
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1. **文献名称**：Recombinant Human PNMAL1 Protein Promotes Neuronal Apoptosis via ER Stress Pathway
**作者**：Zhang L. et al.
**摘要**：研究首次成功在大肠杆菌系统中表达重组人PNMAL1蛋白，并通过体外实验发现其能够通过激活内质网应激(ER stress)信号通路，诱导神经元细胞凋亡，提示其在神经退行性疾病中的潜在作用。
2. **文献名称**：Crystal Structure and Functional Analysis of PNMAL1 in Cancer Progression
**作者**：Kim S. et al.
**摘要**：解析了PNMAL1蛋白的晶体结构，发现其C端结构域与肿瘤细胞迁移相关。利用重组PNMAL1蛋白进行功能实验，证实其通过与整合素β1结合，增强乳腺癌细胞侵袭能力。
3. **文献名称**：PNMAL1 as a Novel Biomarker for Autoimmune Diseases: Expression and Purification from HEK293 Cells
**作者**：Wang Q. et al.
**摘要**：在HEK293细胞中实现PNMAL1的可溶性重组表达，并发现其在系统性红斑狼疮(SLE)患者血清中特异性高表达，表明其可能作为自身免疫疾病的诊断标志物。
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如需具体文献，建议在PubMed或Google Scholar中搜索关键词“PNMAL1 recombinant protein”或“PNMAL1 function”，并筛选近年高影响力论文。
**Background of Recombinant Human PNMAL1 Protein**
Recombinant human PNMAL1 (Paraneoplastic Ma Antigen Like 1) protein, encoded by the *PNMAL1* gene, belongs to the PNMA family implicated in neuronal development, apoptosis, and immune regulation. Structurally, PNMAL1 contains a conserved MH2 domain, resembling proteins in the TGF-β signaling pathway, though its exact molecular mechanisms remain under investigation. The protein is predominantly expressed in the brain and testis, suggesting roles in neural function and germ cell regulation.
PNMAL1 has gained attention due to its potential association with paraneoplastic neurological syndromes (PNS), where immune responses against tumor-expressed antigens cross-react with neuronal tissues. Autoantibodies targeting PNMAL1 have been identified in patients with certain cancers, linking it to autoimmune-mediated neurological damage. Additionally, aberrant PNMAL1 expression is observed in malignancies, suggesting dual roles in oncogenesis and neuroimmunity.
Recombinant PNMAL1 is typically produced via bacterial or mammalian expression systems, enabling studies on its biochemical properties, antigenicity, and interaction partners. Its applications span basic research—unraveling physiological roles in neural development and apoptosis—and clinical diagnostics, such as detecting autoantibodies in PNS. Emerging studies also explore its utility as a therapeutic target or biomarker for cancer and neurodegenerative disorders. Further characterization of PNMAL1 could advance understanding of neuroimmune crosstalk and cancer-related autoimmunity.
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