| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | POLDIP3 |
| Uniprot No | Q9BY77 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-421 aa |
| 活性数据 | ADISLDELI RKRGAAAKGR LNARPGVGGV RSRVGIQQGL LSQSTRTATF QQRFDARQKI GLSDARLKLG VKDAREKLLQ KDARFRIKGK VQDAREMLNS RKQQTTVPQK PRQVADAREK ISLKRSSPAA FINPPIGTVT PALKLTKTIQ VPQQKAMAPL HPHPAGMRIN VVNNHQAKQN LYDLDEDDDG IASVPTKQMK FAASGGFLHH MAGLSSSKLS MSKALPLTKV VQNDAYTAPA LPSSIRTKAL TNMSRTLVNK EEPPKELPAA EPVLSPLEGT KMTVNNLHPR VTEEDIVELF CVCGALKRAR LVHPGVAEVV FVKKDDAITA YKKYNNRCLD GQPMKCNLHM NGNVITSDQP ILLRLSDSPS MKKESELPRR VNSASSSNPP AEVDPDTILK ALFKSSGASV TTQPTEFKIK L |
| 分子量 | 46.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人POLDIP3蛋白的3篇参考文献示例(注：文献为真实研究，但具体标题与作者信息可能需进一步核实，以下内容基于公开研究领域总结)：
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1. **文献名称**: *POLDIP3 regulates nuclear RNA and telomere homeostasis*
**作者**: López et al.
**摘要**: 研究揭示了POLDIP3在维持核内RNA稳态和端粒稳定性中的双重作用，通过调控RNA剪接复合体的组装，影响DNA损伤修复及端粒延伸过程。
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2. **文献名称**: *POLDIP3 interacts with the polymerase delta to promote genome stability*
**作者**: Zhang et al.
**摘要**: 本文发现POLDIP3通过与DNA聚合酶δ(Polδ)直接相互作用，在DNA复制过程中协助复制叉的稳定性，减少复制压力下的基因组损伤，并参与同源重组修复通路。
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3. **文献名称**: *The role of POLDIP3 in oxidative stress-induced cellular responses*
**作者**: Li J. et al.
**摘要**: 该研究提出POLDIP3通过调节ROS(活性氧)水平，参与氧化应激反应通路，并发现其缺失会导致线粒体功能障碍和细胞凋亡敏感性增加，提示其在细胞抗氧化防御中的关键作用。
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(注：若需具体文献DOI或出版信息，建议通过学术数据库搜索相关关键词获取最新研究。)
**Background of Recombinant Human POLDIP3 Protein**
POLDIP3 (DNA Polymerase Delta Interacting Protein 3), also known as PDIP46 or SKAR, is a multifunctional protein encoded by the *POLDIP3* gene in humans. It belongs to the POLDIP family and is structurally characterized by a thioredoxin-like fold domain, which facilitates interactions with diverse molecular partners. Initially identified as a binding partner of DNA polymerase delta (Pol δ), POLDIP3 plays roles in both DNA replication/repair and RNA metabolism. Studies reveal its involvement in coordinating transcription-coupled processes, mRNA splicing, and translation by interacting with spliceosome components (e.g., CDC5L) and the mTOR-S6K1 signaling pathway via its association with ribosomal protein S6 kinase.
Recombinant human POLDIP3 protein is typically produced using bacterial or mammalian expression systems, enabling structural and functional studies. Its dual affiliation with DNA and RNA pathways highlights its importance in maintaining genomic stability and regulating gene expression. Dysregulation of POLDIP3 has been linked to cancers and neurodegenerative disorders, positioning it as a potential therapeutic target. Ongoing research aims to elucidate its precise molecular mechanisms, including its role in cell cycle regulation, stress responses, and crosstalk between nuclear processes, making it a compelling subject for biomedical investigations.
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