| 纯度 | ＞90% by SDS-PAGE. |
| 种属 | Human |
| 靶点 | ACAA1 |
| Uniprot No | P09110 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 27-424aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMLSGAPQASAADVVVVHGRRTAICRAGRGG FKDTTPDELLSAVMTAVLKDVNLRPEQLGDICVGNVLQPGAGAIMARIAQ FLSDIPETVPLSTVNRQCSSGLQAVASIAGGIRNGSYDIGMACGVESMSL ADRGNPGNITSRLMEKEKARDCLIPMGITSENVAERFGISREKQDTFALA SQQKAARAQSKGCFQAEIVPVTTTVHDDKGTKRSITVTQDEGIRPSTTME GLAKLKPAFKKDGSTTAGNSSQVSDGAAAILLARRSKAEELGLPILGVLR SYAVVGVPPDIMGIGPAYAIPVALQKAGLTVSDVDIFEINEAFASQAAYC VEKLRLPPEKVNPLGGAVALGHPLGCTGARQVITLLNELKRRGKRAYGVV SMCIGTGMGAAAVFEYPGN |
| 预测分子量 | 44 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ACAA1重组蛋白的3篇参考文献示例(基于真实研究背景，文献标题和作者为虚构，内容概括参考相关领域研究)：
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1. **文献名称**: *"Recombinant Expression and Functional Characterization of Human ACAA1 in Escherichia coli"*
**作者**: Li, X., Wang, Y., & Chen, J.
**摘要**: 本研究成功在大肠杆菌中表达了重组人源ACAA1蛋白，并通过亲和层析纯化获得高纯度产物。酶活实验表明，重组ACAA1具有硫解酶活性，可催化脂肪酸β-氧化中的关键步骤，为后续代谢疾病研究提供了工具。
2. **文献名称**: *"Structural Insights into ACAA1 Through Crystallography of Recombinant Protein"*
**作者**: Müller, R., et al.
**摘要**: 通过重组表达ACAA1并解析其晶体结构，揭示了该蛋白的活性位点构象及底物结合机制。研究发现，ACAA1的催化残基与辅酶A的结合模式与其他硫解酶家族成员存在差异，提示其独特的底物特异性。
3. **文献名称**: *"ACAA1 Deficiency and Lipid Metabolism: Role of Recombinant Protein in Cellular Models"*
**作者**: Gonzalez, A., et al.
**摘要**: 利用重组ACAA1蛋白在ACAA1缺陷细胞系中进行功能回补实验，证实其参与调控细胞内脂质积累。研究表明，ACAA1表达水平与线粒体脂肪酸氧化能力呈正相关，为相关代谢综合征提供了分子机制解释。
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**注**：以上文献为示例，实际研究中建议通过PubMed或Web of Science以关键词“ACAA1 recombinant”“ACAA1 expression”检索最新文献。真实存在的相关研究可能包括ACAA1在肿瘤代谢、神经退行性疾病中的功能探索。
**Background of ACAA1 Recombinant Protein**
ACAA1 (acetyl-CoA acyltransferase 1), also known as peroxisomal thiolase 1. is a key enzyme involved in lipid metabolism, specifically in the β-oxidation pathway. It catalyzes the final step of peroxisomal fatty acid degradation, cleaving 3-ketoacyl-CoA into acetyl-CoA and a shortened acyl-CoA molecule. This reaction is critical for energy production, biosynthesis of phospholipids, and detoxification of very long-chain fatty acids (VLCFAs). ACAA1 is predominantly localized in peroxisomes, organelles essential for breaking down complex lipids, and its dysfunction is linked to metabolic disorders, such as peroxisomal biogenesis defects and Zellweger spectrum disorders.
Recombinant ACAA1 protein is engineered using heterologous expression systems (e.g., *E. coli* or mammalian cells*) to produce purified, functional enzyme for research. Its recombinant form retains catalytic activity, enabling studies on substrate specificity, enzyme kinetics, and interactions with cofactors. Researchers utilize ACAA1 recombinant protein to investigate peroxisomal disorders, model metabolic pathways, and screen potential therapeutic compounds targeting lipid metabolism anomalies. Additionally, it serves as a tool to explore ACAA1's role in cellular processes beyond β-oxidation, including inflammation and oxidative stress regulation.
The development of recombinant ACAA1 has advanced structural biology efforts, revealing insights into its homodimeric architecture and active-site mechanisms. Such studies are pivotal for understanding disease-causing mutations and designing enzyme replacement strategies. Overall, ACAA1 recombinant protein is a vital resource for dissecting lipid metabolism intricacies and developing interventions for related disorders.
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