| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PRR25 |
| Uniprot No | Q96S07 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-402 aa |
| 活性数据 | MARTDQKPPC RGGCWGQPGH PNTGGAAAHP TYHPMGHRPR TCILLRGDQT TGGQAPSREI SLGPWAAGTH FLAISTTPWG RKTPACISEL PTSSGTAQPL ANAVCEVQTV PGPGLRPQGT PAMRAPSHKG TPPTPNPWGP EQPQNRHKHP KKGVTGGPSP PPPAASRYGQ TPGREPRVQA PGLGPCGRPA SGRLLSLHLE KGDGKGTRQR IPLTDAAVGG DRTDIPSAIA AGPARTPDRH GLPIPGSTPT PMVGSGRLGA PVGRSGGGAS ARSSRPSCAN VLLRADASLG TVLSVLWTGQ LSRGWALLPP GDAGRHLETS VISAGVAAGI WLVEPGEAAQ DPATRRTAPP RRTASPEPPA PGAPLPACPG RIPGAARFGP RSCPLGSPAV LAVTTGWSHR SV |
| 分子量 | 40.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人PRR25蛋白的3篇参考文献的简要信息(注：PRR25研究较少，以下为模拟示例)：
1. **文献名称**: "Proline-rich protein 25 regulates neural stem cell proliferation"
**作者**: Zhang L, et al.
**摘要**: 报道了PRR25在小鼠神经干细胞中的表达及功能，通过重组蛋白实验发现其过表达可抑制细胞周期进程。
2. **文献名称**: "Crystal structure of human PRR25 proline-rich domain"
**作者**: Watanabe K, et al.
**摘要**: 解析了PRR25蛋白的脯氨酸富集区晶体结构，揭示了其与SH3结构域相互作用的潜在机制。
3. **文献名称**: "PRR25 modulates Wnt signaling in colorectal cancer"
**作者**: Chen X, et al.
**摘要**: 利用重组PRR25蛋白进行体外实验，证明其通过结合β-catenin调控Wnt通路活性，影响结直肠癌细胞迁移。
注：真实文献中PRR25研究极为有限(截至2023年)，建议确认基因名称准确性或扩大检索范围至相关家族蛋白研究。如需具体文献，可提供基因全称或研究背景进一步分析。
Recombinant human PRR25 (proline-rich protein 25) is a poorly characterized protein encoded by the PRR25 gene, located on chromosome 9q32 in humans. Structurally, PRR25 contains multiple proline-rich domains, a feature often associated with protein-protein interactions and signaling pathway regulation. While its exact biological function remains unclear, bioinformatic analyses suggest potential roles in cellular processes such as transcriptional regulation or stress response. The protein's expression appears tissue-specific, with limited mRNA detection in normal human tissues, though its dysregulation has been sporadically reported in certain cancer studies. Recombinant PRR25 is typically produced using bacterial (e.g., E. coli) or mammalian expression systems, often fused with tags like His-tag for purification. This engineered form enables preliminary functional studies, antibody development, and interaction partner screening. Current research challenges include clarifying its subcellular localization, physiological ligands, and mechanistic contributions to cellular pathways. The lack of comprehensive studies and standardized nomenclature (PRR25 is occasionally referenced as C9orf131 in older databases) contributes to its enigmatic status in proteomic research. Further investigation is required to validate proposed links to human diseases and assess its potential as a therapeutic target or diagnostic biomarker.
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