| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RAB36 |
| Uniprot No | O95755 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-333 aa |
| 活性数据 | MVIAGASWML GRAAASPTQT PPTTSTIRVA RRSRVALVAM VIAAAGSGGP GRAEPQLSQP SLDCGRMRSS LTPLGPPVSR DRVIASFPKW YTPEACLQLR EHFHGQVSAA CQRRNTGTVG LKLSKVVVVG DLYVGKTSLI HRFCKNVFDR DYKATIGVDF EIERFEIAGI PYSLQIWDTA GQEKFKCIAS AYYRGAQVII TAFDLTDVQT LEHTRQWLED ALRENEAGSC FIFLVGTKKD LLSGAACEQA EADAVHLARE MQAEYWSVSA KTGENVKAFF SRVAALAFEQ SVLQDLERQS SARLQVGNGD LIQMEGSPPE TQESKRPSSL GCC |
| 分子量 | 29.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人RAB36蛋白的假设性参考文献示例(可能非真实文献，供格式参考)：
---
1. **文献名称**：RAB36 regulates lipid droplet dynamics in adipocytes
**作者**：Zhang et al.
**摘要**：本研究揭示RAB36通过调控脂滴与内体系统的膜融合，影响脂肪细胞的脂质存储。重组人RAB36蛋白的体外实验表明其特异性结合脂滴相关蛋白，并促进脂肪生成。
2. **文献名称**：RAB36 modulates autophagosome-lysosome fusion in neurodegenerative diseases
**作者**：Liu et al.
**摘要**：通过重组RAB36蛋白及基因敲除模型，发现RAB36通过结合LC3和STX17促进自噬体-溶酶体融合障碍的改善，为阿尔茨海默病的治疗提供潜在靶点。
3. **文献名称**：Overexpression of RAB36 promotes cancer cell invasion via EGFR trafficking
**作者**：Tanaka et al.
**摘要**：在肺癌细胞中，重组RAB36蛋白的过表达通过加速EGFR的细胞膜运输增强下游MAPK信号通路，导致肿瘤转移能力显著增加。
4. **文献名称**：RAB36 interacts with the secretory pathway in Golgi apparatus reorganization
**作者**：Chen et al.
**摘要**：利用重组RAB36蛋白的体外囊泡运输模型，证实其通过调控高尔基体相关RabGAP蛋白影响分泌途径，揭示其在细胞器动态平衡中的关键作用。
---
**说明**：以上文献为假设示例，实际文献请通过PubMed、Google Scholar等数据库检索关键词“RAB36 recombinant protein”或联系领域专家获取。真实研究多关注RAB36在囊泡运输、代谢疾病及癌症中的分子机制。
Recombinant human RAB36 protein is a member of the RAB GTPase family, which plays critical roles in regulating intracellular membrane trafficking, vesicle transport, and organelle dynamics. RAB36. specifically, is implicated in specialized cellular processes such as secretory pathways, granule release, and membrane trafficking, potentially influencing autophagy or signaling cascades. It cycles between GTP-bound (active) and GDP-bound (inactive) states, interacting with effector proteins to coordinate cargo sorting, transport, and fusion events. While less characterized than other RAB family members, emerging studies suggest its involvement in neuronal function, lipid metabolism, and immune responses. Dysregulation of RAB36 has been tentatively linked to pathologies like neurodegeneration, cancer metastasis, and metabolic disorders, though mechanistic insights remain limited.
Recombinant RAB36 is typically produced using heterologous expression systems (e.g., E. coli, mammalian cells) with purification techniques ensuring proper folding and post-translational modifications. Its recombinant form enables functional studies, including GTPase activity assays, protein interaction mapping, and subcellular localization analyses. Researchers employ it to elucidate RAB36's role in vesicular transport pathways, organelle crosstalk, and disease mechanisms, offering potential for therapeutic targeting. As a tool, it aids in identifying binding partners, deciphering regulatory networks, and validating cellular phenotypes in knockout or overexpression models. Continued exploration of RAB36 may uncover novel mechanisms underlying membrane trafficking biology and its intersection with human diseases.
×
