| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SARA1 |
| Uniprot No | Q9NR31 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-198 aa |
| 活性数据 | MSFIFEWIYNGFSSVLQFLGLYKKSGKLVFLGLDNAGKTTLLHMLKDDRLGQHVPTLHPTSEELTIAGMTFTTFDLGGHEQARRVWKNYLPAINGIVFLVDCADHSRLVESKVELNALMTDETISNVPILILGNKIDRTDAISEEKLREIFGLYGQTTGKGNVTLKELNARPMEVFMCSVLKRQGYGEGFRWLSQYID |
| 分子量 | 49.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为整理的有关重组人SARA1蛋白的模拟参考文献(基于学术文献常见主题生成，非真实存在)：
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1. **文献名称**: *Structural Insights into SARA1 Protein in TGF-β Signaling Through Recombinant Expression*
**作者**: Chen L. et al.
**摘要**: 研究利用大肠杆菌系统成功表达并纯化重组人SARA1蛋白，通过X射线晶体学解析其三维结构，揭示其与Smad2/3的结合机制，为靶向TGF-β信号通路的药物设计提供依据。
2. **文献名称**: *Functional Role of Recombinant SARA1 in Cancer Metastasis Regulation*
**作者**: Gupta S. et al.
**摘要**: 通过体外实验证明，重组人SARA1蛋白可抑制TGF-β诱导的EMT(上皮间质转化)过程，降低乳腺癌细胞侵袭性，提示其作为肿瘤转移治疗的潜在靶点。
3. **文献名称**: *High-Yield Production of Recombinant SARA1 in Mammalian Cells and Interaction Analysis*
**作者**: Wang Y. et al.
**摘要**: 开发HEK293细胞高效表达系统生产功能性SAR1A蛋白，结合免疫共沉淀技术验证其与内体膜蛋白的结合特性，阐明了其在细胞膜受体运输中的调控作用。
4. **文献名称**: *SARA1 Knockdown and Recombinant Protein Rescue in Fibrotic Disease Models*
**作者**: Rodriguez P. et al.
**摘要**: 在肺纤维化模型中，外源添加重组人SARA1蛋白逆转了Smad7的异常降解，抑制成纤维细胞活化，表明其在纤维化疾病中的治疗潜力。
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**注**: 以上文献为模拟示例，实际研究需参考真实数据库(如PubMed、Web of Science)获取准确信息。建议结合关键词“SAR1A (Sar1b)”、“TGF-beta SARA”或“recombinant SAR1A production”进一步检索。
**Recombinant Human SARA1 Protein: Background and Significance**
The **SARA1 (Smad Anchor for Receptor Activation)** protein plays a pivotal role in the **TGF-β (Transforming Growth Factor-beta) signaling pathway**, a critical regulator of cell proliferation, differentiation, and apoptosis. SARA1 acts as an adaptor protein, facilitating the recruitment of **Smad2/3 transcription factors** to activated TGF-β receptors at early endosomes, thereby enabling their phosphorylation and subsequent nuclear translocation to modulate gene expression.
Structurally, SARA1 contains a **FYVE domain** that binds phosphatidylinositol 3-phosphate (PI3P), directing its localization to endosomal membranes. This spatial regulation ensures precise signal transduction. Recombinant human SARA1 protein, produced via *in vitro* expression systems (e.g., *E. coli* or mammalian cells), retains these functional domains, enabling researchers to study SARA1’s interactions, receptor-Smad complex formation, and pathway modulation in controlled settings.
Applications of recombinant SARA1 include **mechanistic studies** of TGF-β signaling dysregulation linked to fibrosis, cancer, and immune disorders. It also serves as a tool for **drug discovery**, screening molecules that target Smad-dependent pathways. By elucidating SARA1’s role in signal specificity and crosstalk, this recombinant protein advances our understanding of cellular responses to TGF-β and potential therapeutic interventions.
In summary, recombinant SARA1 is indispensable for dissecting TGF-β pathway mechanics and developing treatments for related diseases.
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