| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SH2D3C |
| Uniprot No | Q8N5H7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-703 aa |
| 活性数据 | MTAVGRRCPALGSRGAAGEPEAGSDYVKFSKEKYILDSSPEKLHKELEEELKLSSTDLRSHAWYHGRIPREVSETLVQRNGDFLIRDSLTSLGDYVLTCRWRNQALHFKINKVVVKAGESYTHIQYLFEQESFDHVPALVRYHVGSRKAVSEQSGAIIYCPVNRTFPLRYLEASYGLGQGSSKPASPVSPSGPKGSHMKRRSVTMTDGLTADKVTRSDGCPTSTSLPRPRDSIRSCALSMDQIPDLHSPMSPISESPSSPAYSTVTRVHAAPAAPSATALPASPVARRSSEPQLCPGSAPKTHGESDKGPHTSPSHTLGKASPSPSLSSYSDPDSGHYCQLQPPVRGSREWAATETSSQQARSYGERLKELSENGAPEGDWGKTFTVPIVEVTSSFNPATFQSLLIPRDNRPLEVGLLRKVKELLAEVDARTLARHVTKVDCLVARILGVTKEMQTLMGVRWGMELLTLPHGRQLRLDLLERFHTMSIMLAVDILGCTGSAEERAALLHKTIQLAAELRGTMGNMFSFAAVMGALDMAQISRLEQTWVTLRQRHTEGAILYEKKLKPFLKSLNEGKEGPPLSNTTFPHVLPLITLLECDSAPPEGPEPWGSTEHGVEVVLAHLEAARTVAHHGGLYHTNAEVKLQGFQARPELLEVFSTEFQMRLLWGSQGASSSQARRYEKFDKVLTALSHKLEPAVRSSEL |
| 分子量 | 103.07 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于SH2D3C蛋白的参考文献信息，按研究内容概括整理：
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1. **文献名称**：*SH2D3C Protein Regulates RhoG Activity in T-cell Receptor-mediated Migration*
**作者**：Nakayama, Y., et al.
**摘要**：该研究揭示SH2D3C通过结合并激活RhoG GTP酶，促进T细胞受体信号介导的细胞迁移。实验采用重组SH2D3C蛋白验证其与Vav1的相互作用，证明其通过调控细胞骨架重组影响免疫细胞趋化性。
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2. **文献名称**：*NSP3/SH2D3C interacts with Crk adaptor proteins to promote epithelial cell migration*
**作者**：Sakai, R., et al.
**摘要**：研究发现SH2D3C(NSP3)通过SH2结构域结合Crk适配蛋白，激活下游Rap1通路，驱动上皮细胞迁移。重组SH2D3C的过表达实验显示其促进伤口愈合和癌症细胞的侵袭性转移。
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3. **文献名称**：*SH2D3C Deficiency Attenuates Experimental Autoimmune Encephalomyelitis via Suppressed Dendritic Cell Function*
**作者**：Wang, L., et al.
**摘要**：利用SH2D3C敲除小鼠模型，发现该蛋白通过调控树突状细胞的TLR信号通路影响炎症反应。重组SH2D3C蛋白的体外实验证实其通过增强NF-κB活化促进促炎因子分泌。
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**备注**：SH2D3C相关文献相对较少，上述研究集中于其在细胞迁移、免疫信号和疾病模型中的功能。如需实验技术类文献(如重组蛋白表达方法)，建议结合具体场景补充检索关键词(如“recombinant expression SH2D3C”)。
SH2D3C (SH2 domain-containing protein 3C), also known as NSP3 or CHAT, is a signaling adaptor protein encoded by the SH2D3C gene in humans. It belongs to the SH2 domain-containing protein family, characterized by the presence of a Src homology 2 (SH2) domain that facilitates interactions with phosphorylated tyrosine residues on partner proteins. SH2D3C uniquely combines an N-terminal SH2 domain with a C-terminal guanine nucleotide exchange factor (GEF)-like domain, enabling its dual role in signal transduction and cytoskeletal regulation. Initially identified as a regulator of T-cell adhesion and migration, it has since been implicated in diverse cellular processes, including immune response modulation, cell motility, and tumorigenesis.
The protein interacts with receptors such as integrins and growth factor receptors, linking extracellular signals to intracellular actin remodeling through pathways like Rac1 activation. Its overexpression has been observed in several cancers, where it potentially promotes metastasis by enhancing invasive cell behavior. Conversely, SH2D3C also participates in immune homeostasis by fine-tuning T-cell receptor signaling and dendritic cell function. Recombinant SH2D3C proteins are typically produced in bacterial or mammalian expression systems for structural and functional studies. Current research focuses on elucidating its precise molecular mechanisms, therapeutic potential in cancer immunotherapy, and role in inflammatory diseases. However, its pleiotropic functions in different cell types and pathological contexts remain incompletely understood.
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