| 纯度 | ＞90% by SDS-PAGE. |
| 种属 | Human |
| 靶点 | ACAA2 |
| Uniprot No | P42765 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 17-397aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSFGAYGGLLKDFTATDLSEFAAKAALSA GKVSPETVDSVIMGNVLQSSSDAIYLARHVGLRVGIPKETPALTINRLCG SGFQSIVNGCQEICVKEAEVVLCGGTESMSQAPYCVRNVRFGTKLGSDIK LEDSLWVSLTDQHVQLPMAMTAENLAVKHKISREECDKYALQSQQRWKAA NDAGYFNDEMAPIEVKTKKGKQTMQVDEHARPQTTLEQLQKLPPVFKKDG TVTAGNASGVADGAGAVIIASEDAVKKHNFTPLARIVGYFVSGCDPSIMG IGPVPAISGALKKAGLSLKDMDLVEVNEAFAPQYLAVERSLDLDISKTNV NGGAIALGHPLGGSGSRITAHLVHELRRRGGKYAVGSACIGGGQGIAVII QSTA |
| 预测分子量 | 43 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ACAA2重组蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**: *"Functional Characterization of Recombinant Human ACAA2 Enzyme in Peroxisomal Lipid Metabolism"*
**作者**: Smith, J. et al.
**摘要**: 本研究成功在大肠杆菌中表达了重组人源ACAA2蛋白，并验证其硫解酶活性。结果表明，ACAA2在过氧化物酶体β-氧化途径中催化长链脂肪酸分解，且在遗传性代谢紊乱疾病中突变可能影响其功能。
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2. **文献名称**: *"Crystal Structure and Substrate Specificity of Recombinant ACAA2: Implications for Mitochondrial Energy Metabolism"*
**作者**: Li, X. & Wang, Y.
**摘要**: 通过解析重组ACAA2蛋白的晶体结构，揭示了其底物结合口袋的关键氨基酸残基。酶动力学实验表明，ACAA2优先作用于中链脂肪酸衍生物，提示其在调节线粒体能量代谢中的独特作用。
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3. **文献名称**: *"Development of a High-Throughput Assay for ACAA2 Activity Using Recombinant Protein: Application in Drug Screening"*
**作者**: Gupta, R. et al.
**摘要**: 研究团队建立了一种基于重组ACAA2蛋白的高通量酶活检测方法，用于筛选调控脂肪酸氧化的化合物。该方法在代谢疾病药物开发中展现出高灵敏度和可重复性。
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注：以上文献信息为示例性质，实际引用时需核实真实文献来源。建议通过PubMed或Web of Science以“ACAA2 recombinant”为关键词检索最新研究。
**Background of ACAA2 Recombinant Protein**
ACAA2 (Acetyl-CoA Acyltransferase 2), also known as thiolase 2. is a mitochondrial enzyme encoded by the *ACAA2* gene located on chromosome 19q13.2. It belongs to the thiolase family, which catalyzes the final step of mitochondrial fatty acid β-oxidation, a critical pathway for energy production. Specifically, ACAA2 facilitates the cleavage of 3-ketoacyl-CoA into acetyl-CoA and a shortened acyl-CoA, enabling the breakdown of long-chain fatty acids. This process is essential for maintaining cellular energy homeostasis, particularly in tissues with high metabolic demands, such as the liver, heart, and skeletal muscle.
Recombinant ACAA2 protein is engineered using biotechnological methods, often expressed in *E. coli* or mammalian cell systems, to ensure proper folding and enzymatic activity. Its production enables detailed study of ACAA2’s structure, function, and regulatory mechanisms. Researchers employ this tool to investigate mutations or dysregulation linked to metabolic disorders, including rare genetic diseases like mitochondrial thiolase deficiency, which disrupts fatty acid metabolism and can lead to severe neurological, hepatic, or cardiac complications.
Furthermore, ACAA2 has been implicated in broader physiological contexts, such as insulin resistance, cancer metabolism, and neurodegenerative diseases, where altered lipid metabolism plays a role. Recombinant ACAA2 is also used in drug discovery to screen for modulators of thiolase activity, offering potential therapeutic avenues. Studies often pair it with proteomic or metabolomic analyses to map its interactome or downstream metabolic effects.
Despite its established role, ACAA2's regulatory networks and tissue-specific functions remain under exploration. The availability of recombinant ACAA2 continues to advance understanding of mitochondrial biology and its implications in human health and disease.
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