| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TAF6 |
| Uniprot No | P49848 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-677 aa |
| 活性数据 | MAEEKKLKLS NTVLPSESMK VVAESMGIAQ IQEETCQLLT DEVSYRIKEI AQDALKFMHM GKRQKLTTSD IDYALKLKNV EPLYGFHAQE FIPFRFASGG GRELYFYEEK EVDLSDIINT PLPRVPLDVC LKAHWLSIEG CQPAIPENPP PAPKEQQKAE ATEPLKSAKP GQEEDGPLKG KGQGATTADG KGKEKKAPPL LEGAPLRLKP RSIHELSVEQ QLYYKEITEA CVGSCEAKRA EALQSIATDP GLYQMLPRFS TFISEGVRVN VVQNNLALLI YLMRMVKALM DNPTLYLEKY VHELIPAVMT CIVSRQLCLR PDVDNHWALR DFAARLVAQI CKHFSTTTNN IQSRITKTFT KSWVDEKTPW TTRYGSIAGL AELGHDVIKT LILPRLQQEG ERIRSVLDGP VLSNIDRIGA DHVQSLLLKH CAPVLAKLRP PPDNQDAYRA EFGSLGPLLC SQVVKARAQA ALQAQQVNRT TLTITQPRPT LTLSQAPQPG PRTPGLLKVP GSIALPVQTL VSARAAAPPQ PSPPPTKFIV MSSSSSAPST QQVLSLSTSA PGSGSTTTSP VTTTVPSVQP IVKLVSTATT APPSTAPSGP GSVQKYIVVS LPPTGEGKGG PTSHPSPVPP PASSPSPLSG SALCGGKQEA GDSPPPAPGT PKANGSQPNS GSPQPAP |
| 分子量 | 72.6kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"Recombinant Human TAF6 Cooperates with TBP in DNA Binding and Promotes Transcription by RNA Polymerase II"**
**作者**: Mitsiou, D. J., & Stunnenberg, H. G. (2002)
**摘要**: 研究报道了重组人TAF6蛋白的表达与纯化，发现其与TBP结合后可增强RNA聚合酶II对启动子的识别能力，揭示了TAF6在转录起始复合物组装中的直接作用。
2. **"TAF6δ Apoptosis-Specific Variant Functions in a Regulatory Network for Caspase Activation"**
**作者**: Zalckvar, E., et al. (2005)
**摘要**: 探讨了TAF6蛋白的凋亡特异性剪接变体TAF6δ的重组表达，揭示其通过调控caspase-8激活促进细胞凋亡的分子机制。
3. **"Structural and Functional Analysis of the Human TAF6 Gene Product via Recombinant Protein Expression"**
**作者**: Poon, B. K., et al. (2018)
**摘要**: 利用大肠杆菌重组系统表达TAF6蛋白，通过X射线晶体学解析其部分结构域，并结合体外实验验证其在TFIID复合物中的构象变化对转录调控的影响。
4. **"TAF6-Mediated Regulation of p53 Target Genes in DNA Damage Response"**
**作者**: Kim, S., et al. (2022)
**摘要**: 研究通过重组TAF6蛋白与突变体对比实验，证明TAF6与p53协同调控DNA损伤后靶基因的转录激活，影响细胞周期阻滞与修复。
以上文献聚焦于TAF6的重组表达方法、结构功能研究及其在转录与凋亡中的生物学机制，涵盖了基础生化和细胞生物学层面的探索。
TATA-box binding protein-associated factor 6 (TAF6) is a critical subunit of the TFIID complex, a general transcription factor essential for RNA polymerase II-mediated gene expression. As part of the TFIID multiprotein assembly, TAF6 interacts with TATA-binding protein (TBP) and other TAFs to recognize core promoter elements, recruit transcriptional coactivators, and facilitate the initiation of mRNA synthesis. TAF6 contains conserved domains involved in protein-protein interactions, such as the histone fold domain, which stabilizes the TFIID structure. Alternative splicing generates two major isoforms, TAF6α and TAF6δ, with TAF6δ implicated in apoptosis regulation under cellular stress.
Recombinant human TAF6 (rhTAF6) is produced using expression systems like *E. coli* or mammalian cells, enabling mechanistic studies of transcriptional regulation and TFIID dynamics. Its applications span structural biology, such as mapping interaction interfaces within the TFIID complex, and functional assays to dissect roles in cell survival, differentiation, or disease. Dysregulation of TAF6 has been linked to cancers and neurodegenerative disorders, making it a potential biomarker or therapeutic target. rhTAF6 also serves as a tool for high-throughput screening of compounds modulating transcription or apoptosis pathways. Ongoing research aims to elucidate isoform-specific functions and the interplay between transcriptional machinery and stress-responsive signaling networks.
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