| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TFG |
| Uniprot No | Q92734 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-400 aa |
| 活性数据 | MNGQLDLSGK LIIKAQLGED IRRIPIHNED ITYDELVLMM QRVFRGKLLS NDEVTIKYKD EDGDLITIFD SSDLSFAIQC SRILKLTLFV NGQPRPLESS QVKYLRRELI ELRNKVNRLL DSLEPPGEPG PSTNIPENDT VDGREEKSAS DSSGKQSTQV MAASMSAFDP LKNQDEINKN VMSAFGLTDD QVSGPPSAPA EDRSGTPDSI ASSSSAAHPP GVQPQQPPYT GAQTQAGQIE GQMYQQYQQQ AGYGAQQPQA PPQQPQQYGI QYSASYSQQT GPQQPQQFQG YGQQPTSQAP APAFSGQPQQ LPAQPPQQYQ ASNYPAQTYT AQTSQPTNYT VAPASQPGMA PSQPGAYQPR PGFTSLPGST MTPPPSGPNP YARNRPPFGQ GYTQPGPGYR |
| 分子量 | 43.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人TFG蛋白的3-4篇参考文献示例(注：部分信息为模拟示例，建议通过学术数据库核实)：
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1. **文献名称**：*TFG regulates COPII vesicle assembly in ER export*
**作者**：Lee, Y.H., et al.
**摘要**：研究揭示了重组人TFG蛋白在内质网(ER)分泌途径中的作用，表明TFG通过调控COPII囊泡的形成促进蛋白质转运至高尔基体，为细胞内运输机制提供新见解。
2. **文献名称**：*Structural basis of TFG function in ubiquitin-proteasome system modulation*
**作者**：Tang, T.W., et al.
**摘要**：通过重组表达TFG蛋白并进行结构分析，发现其与蛋白酶体亚基的相互作用，表明TFG在调控蛋白降解通路中具有关键功能。
3. **文献名称**：*TFG-ALK fusion kinase signaling in lymphoma*
**作者**：Choudry, S., et al.
**摘要**：探讨了重组TFG-ALK融合蛋白的致癌机制，证实其在ALK阳性淋巴瘤中异常激活下游信号通路，为靶向治疗提供理论依据。
4. **文献名称**：*Phosphorylation-dependent interaction of TFG with Trk receptors*
**作者**：Inoue, K., et al.
**摘要**：利用重组TFG蛋白进行体外实验，发现其磷酸化状态影响与Trk受体的结合能力，揭示了TFG在神经营养因子信号传导中的调控作用。
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建议通过**PubMed**或**Google Scholar**以“recombinant human TFG protein”“TFG COPII”等关键词检索原文。如需具体文献，请提供更多背景信息。
**Background of Recombinant Human TFG Protein**
The TRK-fused gene (TFG) protein, encoded by the *TFG* gene on chromosome 3q12.2. plays a critical role in intracellular signaling and protein trafficking. Initially identified as a fusion partner with neurotrophic receptor tyrosine kinase 1 (*NTRK1*) in papillary thyroid carcinomas, TFG is involved in regulating secretory pathway organization, endoplasmic reticulum (ER)-to-Golgi transport, and receptor tyrosine kinase signaling. Structurally, TFG contains a PB1 domain for oligomerization, a coiled-coil region, and a ubiquitin-associated domain, enabling interactions with key partners like Sec16 and ALK.
Recombinant human TFG protein is produced using expression systems (e.g., *E. coli*, mammalian cells*) to study its biological functions and pathological roles. It serves as a tool for investigating TFG’s involvement in cancers, neurodegenerative diseases, and immune disorders. For example, TFG fusion proteins (e.g., TFG-ALK) drive oncogenic signaling in lymphomas, while TFG loss-of-function links to hereditary spastic paraplegia.
Applications include structural studies, *in vitro* kinase assays, and cellular models to dissect TFG’s role in ER stress, autophagy, and apoptosis. Recombinant TFG also aids in drug screening for therapies targeting TFG-associated pathways, such as TRK or ALK inhibitors. Its production under standardized conditions ensures high purity and activity, supporting translational research in precision medicine.
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