| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF396 |
| Uniprot No | Q96N95 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-335 aa |
| 活性数据 | MSAKLGKSSS LLTQTSEECN GILTEKMEEE EQTCDPDSSL HWSSSYSPET FRQQFRQFGY QDSPGPHEAL SRLWELCHLW LRPEVHTKEQ ILELLVLEQF LAILPKELQA WVQKHHPENG EETVTMLEDV ERELDGPKQI FFGRRKDMIA EKLAPSEITE ELPSSQLMPV KKQLQGASWE LQSLRPHDED IKTTNVKSAS RQKTSLGIEL HCNVSNILHM NGSQSSTYRG TYEQDGRFEK RQGNPSWKKQ QKCDECGKIF SQSSALILHQ RIHSGKKPYA CDECAKAFSR SAILIQHRRT HTGEKPYKCH DCGKAFSQSS NLFRHRKRHI RKKVP |
| 分子量 | 38.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ZNF396蛋白的3条参考文献示例(注：文献为假设性描述，实际研究中可能存在差异)：
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1. **文献名称**：*"Identification of ZNF396 as a Novel Regulator of Apoptosis in Human Cells"*
**作者**：Zhang Y, et al.
**摘要**：本研究通过重组表达人ZNF396蛋白，发现其在HeLa细胞中过表达可显著抑制TNF-α诱导的凋亡，并通过RNA测序鉴定出多个凋亡相关基因受其调控，提示ZNF396可能通过表观遗传机制参与细胞存活通路。
2. **文献名称**：*"Structural Insights into the DNA-Binding Domain of ZNF396 and Its Recombinant Production in E. coli"*
**作者**：Tanaka K, et al.
**摘要**：作者成功在大肠杆菌中高效表达并纯化重组ZNF396蛋白的C端锌指结构域，通过X射线晶体学解析其三维结构，揭示了该结构域特异性识别DNA回文序列的分子机制。
3. **文献名称**：*"ZNF396 Knockout Mice Exhibit Neurodevelopmental Deficits and Impaired Synaptic Plasticity"*
**作者**：Wang H, et al.
**摘要**：利用重组ZNF396蛋白体外挽救实验，结合基因敲除小鼠模型，证明ZNF396通过调控BDNF/TrkB信号通路参与神经元突触可塑性，其缺失导致海马依赖的学习记忆能力受损。
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**注意事项**：
- 实际研究中ZNF396相关文献较少，建议通过**PubMed**或**UniProt(ID: Q8WUD4)**获取最新数据。
- 部分研究可能以别名(如ZFP396)发表，需扩展关键词检索。
- 可关注功能关联领域(如转录调控、癌症、神经发育)的交叉研究。
ZNF396 (Zinc Finger Protein 396) is a member of the zinc finger protein family, characterized by conserved C2H2-type zinc finger motifs that facilitate DNA or RNA binding. This protein is encoded by the ZNF396 gene located on human chromosome 7q22.1. Structurally, it contains an N-terminal SCAN domain, implicated in protein-protein interactions, and multiple C-terminal zinc finger motifs that likely mediate sequence-specific nucleic acid recognition. Functionally, ZNF396 is suggested to act as a transcriptional regulator, though its precise biological roles remain under investigation. Studies link it to cancer progression, particularly in hepatocellular carcinoma and glioma, where it may modulate oncogenic pathways such as Wnt/β-catenin signaling. Notably, ZNF396 interacts with the tumor suppressor p53. potentially influencing apoptosis and cell cycle regulation. Recombinant human ZNF396 protein, typically produced in bacterial or mammalian expression systems with affinity tags (e.g., His-tag), enables in vitro studies of its DNA-binding properties, post-translational modifications, and interactome. Its applications span structural analysis, functional assays, and therapeutic target validation, especially in cancer research. However, the protein's full mechanistic landscape and physiological relevance require further exploration.
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