| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF620 |
| Uniprot No | Q6ZNG0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-422 aa |
| 活性数据 | MFQTAWRQEPVTFEDVAVYFTQNEWASLDSVQRALYREVMLENYANVASLAFPFTTPVLVSQLEQGELPWGLDPWEPMGREALRGICPGDEARTEKEGLTPKDHVSKETESFRLMVGGLPGNVSQHLDFGSSLEQPQGHWIIKTKSKRRHFTDTSARHHEAYEVKNGEKFEKLGKNISVSTQLTTNQTNPSGQISYECGQCGRYFIQMADFHRHEKCHTGEKSFECKECGKYFRYNSLLIRHQIIHTGKKPFKCKECGKGLSSDTALIQHQRIHTGEKPYECKECGKAFSSSSVFLQHQRFHTGEKLYECNECWKTFSCSSSFTVHQRMHTGEKPYECKECGKRLSSNTALTQHQRIHTGEKPFECKECGKAFNQKITLIQHQRVHTGEKPYECKVCGKTFSWCGRFILHQKLHTQKTPVQA |
| 分子量 | 73.37 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是基于现有文献特征总结的ZNF620相关研究方向及代表性文献格式参考(注：具体文献需通过学术数据库验证，以下为模拟概括)：
---
1. **标题**: "Cloning and Characterization of Human ZNF620: A Novel C2H2-Type Zinc Finger Protein"
**作者**: Zhang Y, et al.
**摘要**: 该研究首次报道了人源ZNF620基因的克隆及重组蛋白的表达，通过大肠杆菌系统纯化该蛋白，并分析其DNA结合活性，提示其可能参与转录调控。
2. **标题**: "ZNF620 Functions as a Tumor Suppressor in Colorectal Cancer by Regulating p53 Signaling"
**作者**: Lee SM, et al.
**摘要**: 研究发现重组ZNF620蛋白可与p53相互作用并增强其稳定性，通过细胞实验证明其抑制结直肠癌细胞增殖，表明其在癌症中的潜在抑癌作用。
3. **标题**: "Structural Prediction and Functional Analysis of Zinc Finger Protein ZNF620 Using Bioinformatics Tools"
**作者**: Chen X, et al.
**摘要**: 通过生物信息学预测ZNF620的锌指结构域并构建三维模型，推断其可能靶向特定DNA序列，为后续功能研究提供结构基础。
4. **标题**: "ZNF620 Modulates Innate Immune Response by Inhibiting Viral RNA Replication"
**作者**: Wang H, et al.
**摘要**: 研究证明重组ZNF620蛋白能抑制流感病毒复制，机制可能通过结合病毒RNA并激活I型干扰素通路，提示其在抗病毒免疫中的作用。
---
**注意**：以上为示例性内容，实际文献需通过PubMed、Web of Science等平台检索确认。若ZNF620直接研究较少，建议扩展至：
- **锌指蛋白家族**(如ZNFs在基因调控中的共性)
- **结构预测**(AlphaFold预测模型，UniProt数据库ID：Q6ZNB6)
- **相关疾病机制**(如癌症、免疫通路等)
建议使用关键词 **"ZNF620"、"zinc finger protein 620"、"recombinant ZNF620"** 进行专业检索，或联系领域专家获取最新进展。
Zinc finger protein 620 (ZNF620), a member of the Krüppel-associated box (KRAB) domain-containing zinc finger protein family, is encoded by the *ZNF620* gene located on human chromosome 12q13.13. As a transcription factor, it features a conserved C2H2-type zinc finger domain that facilitates sequence-specific DNA binding, along with a KRAB domain at the N-terminus, which mediates protein-protein interactions and transcriptional repression by recruiting chromatin-modifying complexes. ZNF620 is hypothesized to regulate gene expression in cell differentiation, proliferation, or apoptosis, though its precise biological roles remain underexplored.
Studies suggest ZNF620 may function as a tumor-associated protein. It shows differential expression in malignancies such as acute myeloid leukemia and breast cancer, and its downregulation has been linked to poor prognosis in hepatocellular carcinoma. Additionally, ZNF620 may interact with viral proteins, potentially influencing pathogenesis. Recombinant human ZNF620 protein, produced via heterologous expression systems like *E. coli* or mammalian cells, enables functional studies—including DNA-binding assays, interactome mapping, and mechanistic analyses of its regulatory effects. Current research focuses on elucidating its target genes, post-translational modifications, and disease relevance, particularly in cancer and fibrosis. However, structural insights into its zinc finger motifs and context-dependent regulatory mechanisms require further investigation.
×
