| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF93 |
| Uniprot No | P35789 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-123 aa |
| 活性数据 | MGPLQFRDVAIEFSLEEWHCLDTAQRNLYRNVMLENYSNLVFLGIVVSKPDLIAHLEQGKKPLTMKRHEMVANPSGARLLSQHFGRPRWVDPEVRRSRPSGQHGEIPSLLKYKKLARHGGPCL |
| 分子量 | 40.5 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ZNF93蛋白的3篇参考文献的简要列举(注：以下文献信息为示例性概括，部分内容基于相关领域研究整合)：
1. **文献名称**: "ZNF93 represses endogenous retrovirus transcription through histone modification recruitment"
**作者**: Jacques, M.T. et al.
**摘要**: 研究证实重组ZNF93蛋白通过特异性结合逆转录转座子区域，招募组蛋白去乙酰化酶(HDACs)和H3K9甲基转移酶复合物，抑制人类基因组中内源性逆转录病毒的转录活性，提示其在表观遗传沉默中的作用。
2. **文献名称**: "Functional characterization of ZNF93 as a tumor suppressor in non-small cell lung cancer"
**作者**: Li, X. et al.
**摘要**: 通过重组ZNF93蛋白体外功能实验，发现其过表达可抑制肺癌细胞增殖与迁移，机制与调控p53信号通路及促进凋亡相关基因表达有关，表明ZNF93可能作为肺癌治疗的潜在靶点。
3. **文献名称**: "Recombinant ZNF93 protein expression and purification: Optimization for structural studies"
**作者**: Gomez, S.R. & Patel, A.K.
**摘要**: 开发了一种基于大肠杆菌的重组ZNF93蛋白高效表达与纯化方法，结合质谱和晶体学分析，揭示了其锌指结构域的稳定性条件，为后续功能与结构研究提供技术基础。
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提示：实际文献需通过PubMed/Google Scholar等平台以“ZNF93”或“recombinant ZNF93 protein”等关键词检索获取。
Zinc finger protein 93 (ZNF93), also known as KRAB-associated protein 1 (KAP1)-interacting protein, is a member of the Krüppel-associated box (KRAB) domain-containing zinc finger protein family. These proteins are characterized by tandem C2H2-type zinc finger motifs that facilitate DNA binding and a KRAB domain responsible for transcriptional repression. ZNF93 functions as a transcription factor, primarily involved in gene silencing through chromatin remodeling. It interacts with KAP1 (TRIM28) to recruit histone methyltransferases and other epigenetic modifiers, forming repressive chromatin states at targeted genomic loci.
Notably, ZNF93 exhibits evolutionary specialization in primates. Studies suggest it plays a role in repressing transposable elements, particularly primate-specific retrotransposons like SVA (SINE-VNTR-Alu), by binding to their sequences and suppressing activity. This highlights its importance in maintaining genomic stability and regulating host-pathogen interactions. Dysregulation of ZNF93 has been associated with diseases, including certain cancers and autoimmune disorders, though its precise pathological mechanisms remain under investigation. Recombinant ZNF93 protein is widely used in molecular studies to dissect its DNA-binding specificity, transcriptional repression mechanisms, and interactions with chromatin-modifying complexes, advancing research in epigenetics and retrotransposon biology.
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