| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNFN1A5 |
| Uniprot No | Q9H5V7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-100 aa |
| 活性数据 | GEKKPEPLDFVKDFQEYLTQQTHHVNMISGSVSGDKEAEALQGAGTDGDQNGLDHPSVEVSLDENSGMLVDGFERTFDGKLKCRYCNYASKGTARLIEH |
| 分子量 | 36.63 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ZNFN1A5蛋白的参考文献示例(假设性描述，供参考)：
1. **文献名称**: "重组人ZNFN1A5蛋白在大肠杆菌中的高效表达与纯化"
**作者**: 张伟等
**摘要**: 该研究成功构建了ZNFN1A5的重组表达载体，并利用大肠杆菌系统实现可溶性表达。通过镍柱亲和层析纯化，获得高纯度蛋白，并证实其体外DNA结合活性，为后续功能研究奠定基础。
2. **文献名称**: "ZNFN1A5通过调控Wnt信号通路抑制结直肠癌转移"
**作者**: 李明等
**摘要**: 研究利用重组ZNFN1A5蛋白进行细胞功能实验，发现其通过抑制Wnt/β-catenin通路降低癌细胞侵袭能力，提示其可能作为肿瘤治疗的新靶点。
3. **文献名称**: "人ZNFN1A5锌指结构域的三维结构解析"
**作者**: 王芳团队
**摘要**: 通过X射线晶体学解析ZNFN1A5的锌指结构域，揭示其特异性识别DNA基序的分子机制，为设计靶向该蛋白的小分子提供结构基础。
4. **文献名称**: "ZNFN1A5与组蛋白修饰酶的相互作用研究"
**作者**: 陈晨等
**摘要**: 研究发现重组ZNFN1A5蛋白与HDAC1在体外直接结合，并参与染色质重塑，表明其在表观遗传调控中的潜在作用。
**注**：以上文献为假设性示例，实际研究中请确保名称准确性(如ZNFN1A5可能为笔误，建议核对基因官方命名如ZNF385A或其他类似蛋白)。推荐通过PubMed或Google Scholar以正确名称检索相关文献。
Recombinant human ZNFN1A5 (Zinc Finger Protein N1A5) is a genetically engineered protein belonging to the zinc finger transcription factor family, characterized by conserved zinc finger domains that mediate DNA binding. These domains enable ZNFN1A5 to interact with specific nucleic acid sequences, playing a regulatory role in gene expression. Zinc finger proteins are crucial for diverse cellular processes, including development, differentiation, and apoptosis. While the exact biological functions of ZNFN1A5 remain under investigation, homologs in this family are often implicated in transcriptional regulation, chromatin remodeling, and cellular signaling pathways.
Produced via recombinant DNA technology, ZNFN1A5 is typically expressed in bacterial or mammalian systems to ensure proper folding and post-translational modifications. Its recombinant form allows for high purity and consistency, facilitating functional studies. Researchers employ it to explore DNA-protein interactions, gene regulatory mechanisms, and potential roles in diseases such as cancer or genetic disorders. Recent studies suggest zinc finger proteins like ZNFN1A5 may serve as therapeutic targets or tools for gene editing due to their sequence-specific binding capabilities. However, further research is needed to elucidate its precise physiological targets, regulatory networks, and clinical relevance. The protein's versatility in molecular and structural biology continues to drive interest in both basic research and biotechnological applications.
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