| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNHIT2 |
| Uniprot No | Q9UHR6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-403 aa |
| 活性数据 | MEPAGPCGFCPAGEVQPARYTCPRCNAPYCSLRCYRTHGTCAENFYRDQVLGELRGCSAPPSRLASALRRLRQQRETEDEPGEAGLSSGPAPGGLSGLWERLAPGEKAAFERLLSRGEAGRLLPPWRPWWWNRGAGPQLLEELDNAPGSDAAELELAPARTPPDSVKDASAAEPAAAERVLGDVPGACTPVVPTRIPAIVSLSRGPVSPLVRFQLPNVLFAYAHTLALYHGGDDALLSDFCATLLGVSGALGAQQVFASAEEALQAAAHVLEAGEHPPGPLGTRGAMHEVARILLGEGPTNQKGYTLAALGDLAQTLGRARKQAVAREERDHLYRARKKCQFLLAWTNENEAALTPLALDCARAHQAHAVVAEEVAALTGELERLWGGPVPPAPRTLIEELPS |
| 分子量 | 69.3 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ZNHIT2蛋白的3篇文献示例及其摘要概括：
1. **文献名称**：*ZNHIT2 is a novel suppressor of hepatocellular carcinoma by regulating mTORC1-mediated autophagy*
**作者**：Liu Y, et al.
**摘要**：研究发现ZNHIT2在肝癌组织中表达下调，重组ZNHIT2蛋白过表达可通过抑制mTORC1通路激活自噬，抑制肝癌细胞增殖和转移。
2. **文献名称**：*The zinc finger HIT-type protein ZNHIT2 interacts with orphan nuclear receptor NR4A1 to regulate apoptosis*
**作者**：Wang X, et al.
**摘要**：利用重组ZNHIT2蛋白进行互作实验，发现其与核受体NR4A1结合，调控DNA损伤诱导的细胞凋亡，提示其在肿瘤发生中的作用。
3. **文献名称**：*ZNHIT2 mediates p53-dependent genomic stability through histone H2A deposition*
**作者**：Kumar R, et al.
**摘要**：通过重组蛋白体外实验，证明ZNHIT2与SRCAP复合物相互作用，参与组蛋白H2A.Z的染色质定位，维持基因组稳定性并依赖p53通路抑制肿瘤。
(注：上述文献为示例性概括，实际引用需根据具体研究内容查询真实文献。)
**Background of Recombinant Human ZNHIT2 Protein**
Zinc finger HIT-type containing protein 2 (ZNHIT2), also known as HIT2. is a member of the HIT (histone triad) superfamily, characterized by a conserved HIT domain involved in nucleotide binding and hydrolysis. It is encoded by the *ZNHIT2* gene located on human chromosome 11. ZNHIT2 plays critical roles in chromatin remodeling, DNA repair, and transcriptional regulation. Structurally, it contains tandem zinc finger motifs that facilitate interactions with nucleic acids or other proteins, enabling its participation in chromatin-associated complexes.
Recombinant human ZNHIT2 protein is synthesized in vitro using expression systems like *E. coli* or mammalian cell lines, ensuring high purity and activity for functional studies. Research highlights its interaction with nucleosome remodeling complexes, suggesting a role in maintaining genomic stability by mediating DNA damage responses. Dysregulation of ZNHIT2 has been implicated in cancer progression and neurodegenerative disorders, potentially linked to its function in chromatin dynamics. Recent studies also explore its involvement in mitochondrial genome maintenance, expanding its biological significance beyond nuclear processes.
As a research tool, recombinant ZNHIT2 aids in elucidating molecular mechanisms of chromatin regulation and disease pathways, offering potential therapeutic targets. Ongoing investigations aim to clarify its precise molecular partners and downstream signaling cascades, enhancing its relevance in cell biology and medicine.
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