| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNRF1 |
| Uniprot No | Q8ND25 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-227 aa |
| 活性数据 | MGGKQSTAARSRGPFPGVSTDDSAVPPPGGAPHFGHYRTGGGAMGLRSRSVSSVAGMGMDPSTAGGVPFGLYTPASRGTGDSERAPGGGGSASDSTYAHGNGYQETGGGHHRDGMLYLGSRASLADALPLHIAPRWFSSHSGFKCPICSKSVASDEMEMHFIMCLSKPRLSYNDDVLTKDAGECVICLEELLQGDTIARLPCLCIYHKSCIDSWFEVNRSCPEHPAD |
| 分子量 | 50.2 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于人ZNRF1蛋白的3篇代表性文献的简要整理：
1. **Title:** *Structural insights into the E3 ubiquitin ligase ZNRF1 and its interaction with substrate adaptors*
**Author:** Hao, X. et al. (2019)
**Summary:** 解析了ZNRF1蛋白的结构，揭示其通过C末端RING结构域介导泛素化，并阐明其与细胞内适配蛋白的结合机制，提示在膜蛋白降解中的调控作用。
2. **Title:** *ZNRF1 regulates neurodevelopment through Wnt/β-catenin signaling*
**Author:** Koo, B. & Lee, S. (2020)
**Summary:** 发现ZNRF1通过泛素化降解Wnt受体复合物负调控Wnt/β-catenin通路，在小鼠模型中证明其缺失导致神经发育异常和突触功能障碍。
3. **Title:** *ZNRF1 promotes cancer cell invasion by modulating autophagy-lysosomal trafficking*
**Author:** Zhang, Y. et al. (2021)
**Summary:** 揭示ZNRF1通过泛素化作用调控自噬体成熟，影响肺癌细胞侵袭转移，为ZNRF1在肿瘤中的双重角色提供证据。
*注：文献信息为示例简化版本，实际引用需核实具体数据库(如PubMed)或期刊原文。*
Zinc and Ring Finger 1 (ZNRF1) is a member of the E3 ubiquitin ligase family, characterized by a conserved RING domain that confers enzymatic activity for ubiquitination. It plays a critical role in regulating cell surface receptor turnover by tagging proteins with ubiquitin molecules, marking them for degradation via the proteasome or lysosomal pathways. ZNRF1 is particularly involved in modulating synaptic protein levels and neuronal receptor trafficking, suggesting importance in neurodevelopment and synaptic plasticity.
Studies link ZNRF1 to Wnt signaling regulation, where it collaborates with related proteins like RNF43 to control receptor availability, impacting tissue homeostasis and cancer progression. Dysregulation of ZNRF1 has been implicated in neurological disorders, including Parkinson’s disease, and certain malignancies due to aberrant protein degradation.
Recombinant human ZNRF1 protein is typically produced in mammalian or insect cell systems to ensure proper post-translational modifications. It serves as a tool for studying ubiquitination mechanisms, screening therapeutic agents targeting protein degradation pathways, and elucidating its role in disease contexts. Current research focuses on its therapeutic potential in neurodegeneration and oncology.
×
