| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZPBP |
| Uniprot No | Q9BS86 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 45-351 aa |
| 活性数据 | HLVRLPRAFRLTKDSVKIVGSTSFPVKAYVMLHQKSPHVLCVTQQLRNAELIDPSFQWYGPKGKVVSVENRTAQITSTGSLVFQNFEESMSGIYTCFLEYKPTVEEIVKRLQLKYAIYAYREPHYYYQFTARYHAAPCNSIYNISFEKKLLQILSKLLLDLSCEISLLKSECHRVKMQRAGLQNELFFAFSVSSLDTEKGPKRCTDHNCEPYKRLFKAKNLIERFFNQQVEILGRRAEQLPQIYYIEGTLQMVWINRCFPGYGMNVQQHPKCPECCVICSPGSYNPRDGIHCLQCNSSLVYGAKTCL |
| 分子量 | 59.51 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ZPBP蛋白的3篇模拟参考文献示例(请注意，这些为虚构示例，实际文献需通过学术数据库检索确认)：
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1. **文献名称**: *"Cloning and Functional Characterization of Recombinant Human ZPBP in Sperm-Zona Pellucida Interaction"*
**作者**: Smith, J. et al.
**摘要**: 本研究成功克隆并在哺乳动物细胞中表达了重组人ZPBP蛋白，证实其特异性结合人卵母细胞透明带蛋白(如ZP2和ZP3)，为揭示ZPBP在精子受精能力中的作用提供机制依据。
2. **文献名称**: *"Structural Analysis of Recombinant ZPBP Reveals Critical Domains for Sperm-Oocyte Adhesion"*
**作者**: Zhang, L. et al.
**摘要**: 通过X射线晶体学解析重组人ZPBP的三维结构，发现其N端结构域对透明带结合至关重要，敲除该区域显著降低精子穿透透明带的能力，为男性不育诊断提供潜在靶点。
3. **文献名称**: *"Recombinant ZPBP as a Potential Biomarker for Male Infertility Screening"*
**作者**: Gupta, R. et al.
**摘要**: 利用重组人ZPBP开发ELISA检测方法，发现不育男性精浆中ZPBP水平显著低于健康对照组，提示其在临床诊断中的应用价值。
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建议通过PubMed或Google Scholar搜索 **"recombinant human ZPBP"** 或 **"ZPBP sperm function"** 获取真实文献。如需具体文献帮助，可提供更多研究背景进一步缩小范围。
Recombinant human zona pellucida-binding protein (ZPBP) is a biotechnologically engineered version of a naturally occurring sperm protein critical for fertilization. ZPBP, primarily expressed in the testis, is localized on the sperm surface and plays a key role in mediating sperm-zona pellucida (ZP) interaction—a crucial step enabling sperm penetration through the glycoprotein matrix surrounding mammalian oocytes. Structurally, ZPBP contains conserved cysteine-rich domains that facilitate binding to ZP glycoproteins (e.g., ZP2/ZP3) via carbohydrate-mediated recognition. Genetic studies link ZPBP mutations to male infertility, particularly teratozoospermia (abnormal sperm morphology), underscoring its functional importance in spermatogenesis and sperm maturation.
Recombinant ZPBP is typically produced using bacterial or mammalian expression systems, ensuring post-translational modifications that mimic native protein activity. It serves as a vital tool in reproductive biology research, aiding studies on sperm-ZP adhesion mechanisms, contraceptive vaccine development, and diagnostic assays for male infertility. Additionally, recombinant ZPBP has potential clinical applications in improving in vitro fertilization (IVF) outcomes by enhancing sperm selection or evaluating sperm quality. Ongoing research explores its role in sperm capacitation, acrosome reaction, and ZP-induced signaling pathways, further highlighting its dual significance in basic science and translational reproductive medicine.
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