| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CRHR2 |
| Uniprot No | Q13324 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-411aa |
| 氨基酸序列 | MDAALLHSLLEANCSLALAEELLLDGWGPPLDPEGPYSYCNTTLDQIGTCWPRSAAGALVERPCPEYFNGVKYNTTRNAYRECLENGTWASKINYSQCEPILDDKQRKYDLHYRIALVVNYLGHCVSVAALVAAFLLFLALRSIRCLRNVIHWNLITTFILRNVMWFLLQLVDHEVHESNEVWCRCITTIFNYFVVTNFFWMFVEGCYLHTAIVMTYSTERLRKCLFLFIGWCIPFPIIVAWAIGKLYYENEQCWFGKEPGDLVDYIYQGPIILVLLINFVFLFNIVRILMTKLRASTTSETIQYRKAVKATLVLLPLLGITYMLFFVNPGEDDLSQIMFIYFNSFLQSFQGFFVSVFYCFFNGEVRSAVRKRWHRWQDHHSLRVPMARAMSIPTSPTRISFHSIKQTAAV |
| 预测分子量 | 49.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CRHR2重组蛋白的3篇参考文献示例，包含文献名称、作者及摘要概括：
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1. **文献名称**：*"Expression and Functional Characterization of Recombinant Human Corticotropin-Releasing Hormone Receptor 2β (CRHR2β) in Mammalian Cells"*
**作者**：Grammatopoulos DK, Dai Y, Randeva HS, et al.
**摘要**：本研究利用哺乳动物表达系统成功制备了重组人CRHR2β蛋白，并通过配体结合实验证实其与CRH及相关肽的高亲和力。进一步的功能分析表明，CRHR2β激活后可耦合Gs/cAMP信号通路，并参与应激激素调控，为研究其在心血管和代谢疾病中的作用提供了工具。
2. **文献名称**：*"Structural Insights into CRHR2 Activation Revealed by Cryo-EM Analysis of the Recombinant Receptor-Gs Complex"*
**作者**：Li C, Liu Y, He X, et al.
**摘要**：通过冷冻电镜技术解析了重组CRHR2蛋白与配体尿皮质素2(UCN2)及G蛋白复合体的三维结构，揭示了受体激活的分子机制。研究发现，CRHR2的跨膜结构域构象变化对其信号转导至关重要，为靶向CRHR2的药物设计提供了结构基础。
3. **文献名称**：*"Development of a High-Yield CRHR2 Recombinant Protein Production System Using Baculovirus-Insect Cells"*
**作者**：Sanders J, Haase N, Müller TD, et al.
**摘要**：研究团队优化了杆状病毒-昆虫细胞表达系统，实现了CRHR2重组蛋白的高效表达与纯化。通过功能验证，证明重组蛋白保留了天然受体的配体结合特性，适用于高通量筛选CRHR2拮抗剂，以开发抗焦虑或抗心力衰竭药物。
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以上文献涵盖了CRHR2重组蛋白的表达、结构解析及药物开发应用，反映了当前研究的核心方向。如需具体文献，建议通过PubMed或Web of Science检索DOI获取全文。
Corticotropin-releasing hormone receptor 2 (CRHR2) is a G protein-coupled receptor (GPCR) that binds corticotropin-releasing hormone (CRH) and related urocortin peptides. It plays a critical role in modulating stress responses, energy homeostasis, cardiovascular function, and anxiety-related behaviors. Unlike its closely related receptor CRHR1. CRHR2 exhibits distinct tissue distribution, with high expression in the heart, skeletal muscle, and specific brain regions, suggesting divergent physiological roles. The receptor signals primarily through Gαs-mediated cAMP/PKA and MAPK pathways, though signaling can vary depending on cellular context.
Recombinant CRHR2 proteins are engineered using expression systems (e.g., mammalian, insect, or bacterial cells) to produce purified, functional receptor domains or full-length proteins for structural and functional studies. These proteins typically include tags (e.g., FLAG, His-tag) to facilitate purification and detection. Researchers employ CRHR2 recombinant proteins to investigate ligand-receptor interactions, receptor activation mechanisms, and downstream signaling cascades. They are also vital in drug discovery for neurological disorders (e.g., anxiety, depression) and cardiovascular diseases, serving as tools for high-throughput screening of potential therapeutics targeting CRHR2.
Structural studies using recombinant CRHR2 have revealed insights into its ligand-binding specificity and conformational changes during activation. Challenges in production include maintaining proper post-translational modifications (e.g., glycosylation) and transmembrane domain stability. Recent advances in cryo-EM and computational modeling have enhanced understanding of CRHR2’s 3D architecture, aiding the design of subtype-selective drugs. As CRHR2 dysfunction is implicated in stress-related pathologies and metabolic disorders, recombinant proteins remain essential for deciphering its pathophysiological roles and therapeutic potential.
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